Irvine W J, Al-Khateeb S F, Di Mario U, Feek C M, Gray R S, Edmond B, Duncan L J
Clin Exp Immunol. 1977 Oct;30(1):16-21.
Soluble immune complexes were detected by the Raji cell assay in seven out of thirteen newly diagnosed insulin-dependent diabetics, six of whom had islet cell antibodies (ICAb) in the serum. None of the others had ICAb. The titres for a wide range of viral antibodies were similar in these thirteen diabetics as in age- and sex-matched controls, except for antibodies to Coxsackie B4. Six had titres of Coxsackie B4 antibodies greater than or equal to 1:32, but only three of these had evidence of immune complexes in the serum, and these were not correlated with the titres of Coxsackie B4 antibodies. None of these diabetics had antibodies to insulin and none of their age- and sex-matched controls had evidence of immune complexes in the serum. 53% of thirty-two diabetics treated with insulin and 10% of fifty-two diabetics requiring oral hypoglycaemic agents (OHA) or diet had evidence of immune complexes in the serum, compared to 6% of control subjects. High titres of insulin antibodies correlated with evidence for immune complexes. There was a stronger tendency for immune complexes to occur in the presence of moderate titres of insulin antibodies when the age at onset of insulin-dependent diabetes was less than 30 years. Out of sixteen patients treated with heterologous insulin for 13 years or more, and who also had late diabetic complications, twelve had immune complexes in the serum.
采用Raji细胞试验在13例新诊断的胰岛素依赖型糖尿病患者中的7例检测到可溶性免疫复合物，其中6例血清中有胰岛细胞抗体（ICAb），其他患者均无ICAb。除柯萨奇B4病毒抗体外，这13例糖尿病患者的多种病毒抗体滴度与年龄和性别匹配的对照组相似。6例患者的柯萨奇B4病毒抗体滴度大于或等于1:32，但其中只有3例血清中有免疫复合物证据，且这些与柯萨奇B4病毒抗体滴度无关。这些糖尿病患者均无胰岛素抗体，其年龄和性别匹配的对照组血清中也均无免疫复合物证据。与6%的对照受试者相比，32例接受胰岛素治疗的糖尿病患者中有53%以及52例需要口服降糖药（OHA）或饮食控制的糖尿病患者中有10%血清中有免疫复合物证据。高滴度胰岛素抗体与免疫复合物证据相关。当胰岛素依赖型糖尿病发病年龄小于30岁时，在中等滴度胰岛素抗体存在的情况下，免疫复合物出现的倾向更强。在16例接受异体胰岛素治疗13年或更长时间且伴有糖尿病晚期并发症的患者中，12例血清中有免疫复合物。
