用不同示踪剂研究大鼠体内叶酸多聚谷氨酸的生物合成。

Biosynthesis of folate polyglutamate in the rat with different tracers.

Reed B, Weir D G, Scott J M

Clin Sci Mol Med. 1977 Jan;52(1):83-6. doi: 10.1042/cs0520083.

Abstract

The recent suggestions that folate polyglutamate biosynthesis demonstrated in vivo with [3'5'9(n)-3H]folic acid is due to exchange and cannot be achieved with [2(-14)C]folic acid has been demonstrated to be untrue. 2. The compounds formed with both types of radioactive tracer have been shown to be folate polyglutamates from their elution position from precalibrated ion-exchange columns and their susceptibility to hydrolysis by a gamma-carboxypeptidase (conjugase) from human sera. 3. By the use of the methods involving [2(-14)C]folic acid, reported by others to give only folate monoglutamates, we have been able to demonstrate clearly the presence of folate polyglutamates.

摘要

最近有观点认为，用[3'5'9(n)-3H]叶酸在体内证明的叶酸多聚谷氨酸生物合成是由于交换作用，而用[2(-14)C]叶酸无法实现，事实证明这种观点是错误的。2. 用这两种放射性示踪剂形成的化合物，从预先校准的离子交换柱上的洗脱位置以及它们对人血清中的γ-羧肽酶（结合酶）水解的敏感性来看，已表明是叶酸多聚谷氨酸。3. 通过使用其他人报道的仅能产生叶酸单谷氨酸的涉及[2(-14)C]叶酸的方法，我们已能够清楚地证明叶酸多聚谷氨酸的存在。