Romanus M, Stenqvist O, Haljamäe H, Seifert F
Eur Surg Res. 1977;9(6):444-59. doi: 10.1159/000127966.
A model to study pressure-induced ischemia by intravital microscopy is presented. A hamster cheek pouch is prepared to get a single layer of epithelium, together with vessels and connective tissue. Pressure, which can be varied, is transmitted to the tissue by a pressure chamber with a rubber membrane. Microcirculatory reactions may be studied while the pressure is applied, when the pressure is released and as the tissue is regaining circulation. The local environment is controlled by irrigating the test tissue with a solution approximating the composition of interstitial fluid. Local oxygen and carbon dioxide tensions are controlled. There is a marked difference in restoration of blood flow to the tissue after 2- and 4-hour ischemia. After 2-hour ischemia the tissue regained circulation rapidly. Microbleedings developed during the postpressure observation time. After 4-hour ischemia, on the other hand, the tissue regained circulation slowly and only in one third of the microvessels. Extensive white blood cells sticking to the vessel walls were seen indicating endothelial damage. In the 4-hour experiments there were very few microbleedings compared to the 2-hour experiments.
本文介绍了一种通过活体显微镜研究压力诱导缺血的模型。制备仓鼠颊囊以获得单层上皮细胞，以及血管和结缔组织。可调节的压力通过带有橡胶膜的压力室传递至组织。在施加压力时、释放压力时以及组织恢复循环时，均可研究微循环反应。通过用近似组织液成分的溶液冲洗测试组织来控制局部环境。局部氧气和二氧化碳张力也受到控制。在2小时和4小时缺血后，组织血流恢复情况存在显著差异。2小时缺血后，组织迅速恢复循环。在压力后观察期出现微出血。另一方面，4小时缺血后，组织循环恢复缓慢，仅三分之一的微血管恢复循环。可见大量白细胞黏附于血管壁，表明存在内皮损伤。与2小时实验相比，4小时实验中的微出血极少。
