Wellburn A R, Hemming F W
Biochem J. 1967 Jul;104(1):173-7. doi: 10.1042/bj1040173.
Intact chloroplasts and cell walls were prepared from horse-chestnut leaves that had previously metabolized [2-(14)C]mevalonate. The bulk of the castaprenols and plastoquinone-9 was found within the chloroplasts. The remaining portion of the castaprenols was associated with the cell-wall preparation whereas that of the plastoquinone-9 was probably localized in the soluble fraction of the plant cell. The (14)C content of these compounds of different cell fractions indicated the presence of polyisoprenoid-synthesizing activity both inside and outside the chloroplasts. This was confirmed by the relative incorporation of (14)C when ultrasonically treated and intact chloroplasts were incubated with [2-(14)C]mevalonate. As the leaves aged (on the tree) an increase in extraplastidic castaprenols and plastoquinone-9, together with associated synthesizing activities, was observed.
从预先代谢过[2-(14)C]甲羟戊酸的七叶树叶片中制备了完整的叶绿体和细胞壁。发现大部分的角鲨烯醇和质体醌-9存在于叶绿体内。角鲨烯醇的其余部分与细胞壁制剂相关，而质体醌-9的其余部分可能定位于植物细胞的可溶部分。不同细胞组分中这些化合物的(14)C含量表明叶绿体内部和外部均存在多聚异戊二烯合成活性。当用[2-(14)C]甲羟戊酸孵育经超声处理的完整叶绿体时，(14)C的相对掺入证实了这一点。随着叶片（在树上）老化，观察到质体外角鲨烯醇和质体醌-9增加，以及相关的合成活性。
