Morley J S, Capper S J, Miles J
Neuropeptides. 1984 Nov;4(6):477-82. doi: 10.1016/0143-4179(84)90091-x.
Temporary chemical 'protection' of the amino and/or carboxyl termini of small peptides in conjugation reactions with high molecular weight carriers has provided immunogens which give rise to antibodies with high N-terminal and/or C-terminal specificity. Applied to [Leu]enkephalin, the new methodology resulted in antisera of high affinity (K eff 0.1 x 10(12) M-1) which recognized only enkephalin-like pentapeptides. The detailed residue specificity of one enkephalin antiserum is described.
在与高分子量载体的偶联反应中，对小肽的氨基和/或羧基末端进行临时化学“保护”，得到了能产生具有高N端和/或C端特异性抗体的免疫原。将这种新方法应用于亮氨酸脑啡肽，得到了高亲和力（有效解离常数K为0.1×10¹² M⁻¹）的抗血清，该抗血清仅识别脑啡肽样五肽。本文描述了一种脑啡肽抗血清的详细残基特异性。
