Cupo A, Vion-Dury J, Jarry T
Neuropeptides. 1986 Oct;8(3):207-12. doi: 10.1016/0143-4179(86)90048-x.
We described here a new immunization procedure to obtain high titre and high specific antibodies against Leu-enkephalin (LE). The immunogen form is composed of one part of LE conjugate and one part of LE-Arg6 conjugate. We have observed an increase of titre, affinity and specificity of the antibodies in the coimmunization procedure compared to those obtained by conventional immunization involving only the LE conjugate. The Leu-enkephalin antibodies exhibit a high affinity (KD 8 X 10(-12) M) and we are able to detect the Leu-enkephalin at the 10(-15) mole level. These LE antibodies are highly specific of the C part of LE peptide and cross-react weakly with Met-enkephalin (1%).
我们在此描述了一种新的免疫程序，以获得针对亮氨酸脑啡肽（LE）的高滴度和高特异性抗体。免疫原形式由一部分LE缀合物和一部分LE-Arg6缀合物组成。与仅涉及LE缀合物的传统免疫相比，我们观察到在共同免疫程序中抗体的滴度、亲和力和特异性有所增加。亮氨酸脑啡肽抗体表现出高亲和力（KD 8×10⁻¹² M），并且我们能够在10⁻¹⁵摩尔水平检测到亮氨酸脑啡肽。这些LE抗体对LE肽的C部分具有高度特异性，与甲硫氨酸脑啡肽的交叉反应较弱（1%）。
