Gargiulo A M, Ceccarelli P, Pedini V, Moriconi A
Basic Appl Histochem. 1984;28(3):317-27.
The bovine and ovine naso-labial glands have been examined. In both species these glands are multilobular tubulo-acinar, but the cells of secretory units display differences regarding nuclear shape and location, RER arrangement and secretion pattern. In this paper, histochemical characterization of naso-labial gland secretion was performed using the periodic acid Schiff reaction (PAS), the Alcian blue staining (AB) at pH's ranging between 1.7 and 2.5, the Alcian blue-high iron diamine (HID-AB) method, an immunohistochemical staining for muramidase at the light microscopic level and the periodic acid-thiocarbohydrazide-silver proteinate (PA-TCH-SP) technique at the electron microscopic level. In bovines the secretion is composed of glycoproteins, which are reactive to PAS, but not to Alcian blue staining. In ovines, a large quantity of acidic polysaccharides in association with a small quantity of sulfomucins has been found. Ultrastructural and cytochemical observations show that secretory granules do not have a homogeneous structure. They appear to be formed of a meshwork stained positively with the PA-TCH-SP method immersed in a light background. The particular morphological features of the naso-labial glands and their histochemical features suggest that these glands can be classified as sero-mucous in bovines and mucous in ovines.
对牛和羊的鼻唇腺进行了检查。在这两个物种中，这些腺体均为多叶管状腺泡，但分泌单位的细胞在核形状和位置、粗面内质网排列及分泌模式方面存在差异。本文采用过碘酸希夫反应（PAS）、pH值在1.7至2.5之间的阿尔辛蓝染色（AB）、阿尔辛蓝-高铁二胺（HID-AB）法、光镜水平的溶菌酶免疫组织化学染色以及电镜水平的过碘酸-硫代碳酰肼-银蛋白法（PA-TCH-SP）对鼻唇腺分泌物进行组织化学特征分析。在牛中，分泌物由对PAS有反应但对阿尔辛蓝染色无反应的糖蛋白组成。在羊中，发现了大量酸性多糖以及少量硫黏液素。超微结构和细胞化学观察表明，分泌颗粒没有均匀的结构。它们似乎由用PA-TCH-SP法阳性染色的网络构成，沉浸在浅色背景中。鼻唇腺的特殊形态特征及其组织化学特征表明，这些腺体在牛中可归类为浆液性黏液腺，在羊中可归类为黏液腺。
