Kadofuku T, Sato T
J Chromatogr. 1984 Nov 9;311(1):93-9. doi: 10.1016/s0378-4347(00)84694-0.
The changes in rabbit serum proteins after partial hepatectomy were examined by means of two-dimensional electrophoresis utilizing isoelectric focusing in a 4% polyacrylamide gel in the first dimension and a 4-30% pore gradient polyacrylamide gel in the second dimension. A rapid increase in seven proteins was observed after partial hepatectomy and a rapid decrease in two proteins. Major serum proteins, including albumin, immunoglobulin G, immunoglobulin M and alpha 2-macroglobulin, did not change. The time course of the changes was examined using a densitometer; the maxima of the changes were observed on day 3 after partial hepatectomy.
采用二维电泳法研究部分肝切除术后兔血清蛋白质的变化，第一维是在4%聚丙烯酰胺凝胶中进行等电聚焦，第二维是在4%-30%孔径梯度聚丙烯酰胺凝胶中进行。部分肝切除术后观察到7种蛋白质迅速增加，2种蛋白质迅速减少。包括白蛋白、免疫球蛋白G、免疫球蛋白M和α2-巨球蛋白在内的主要血清蛋白质没有变化。使用密度计检查变化的时间进程；在部分肝切除术后第3天观察到变化的最大值。
