Felegie T P, Pasculle A W, Dekker A
J Clin Microbiol. 1984 Dec;20(6):1190-1. doi: 10.1128/jcm.20.6.1190-1191.1984.
In 12 of 20 (60%) biopsy-proven cases of Pneumocystis carinii pneumonia, the diagnosis was first suggested by examination of routine Gram stains of impression smears made from infected lung tissue and later confirmed by methenamine-silver staining. The cysts appeared as 5- to 7-microns unstained spheres, each containing six to eight intracystic gram-negative bodies (sporozoites). Although the Gram stain does not appear to be as sensitive as more traditional staining techniques for the detection of P. carinii, clinical microbiologists should be aware of the morphology of this organism in gram-stained specimens because this relatively simple procedure gives quick results.
在20例经活检证实的卡氏肺孢子虫肺炎病例中，有12例（60%）最初通过检查感染肺组织印片的常规革兰染色提出诊断，随后经亚甲胺银染色得以确诊。囊肿表现为5至7微米的未染色球体，每个球体含有6至8个囊内革兰阴性小体（子孢子）。虽然革兰染色在检测卡氏肺孢子虫方面似乎不如更传统的染色技术敏感，但临床微生物学家应了解该生物体在革兰染色标本中的形态，因为这种相对简单的程序能快速得出结果。
