Rostami A, Brown M J, Lisak R P, Sumner A J, Zweiman B, Pleasure D E
Ann Neurol. 1984 Dec;16(6):680-5. doi: 10.1002/ana.410160610.
Myelin P2 protein has been proposed as the primary antigen in whole myelin-induced experimental allergic neuritis (EAN). We investigated the neuritogenic properties of P2 by sensitizing Lewis rats with complete Freund's adjuvant (CFA) containing P2, P2 plus phosphatidyl serine, or whole myelin containing an equivalent amount of P2. Animals were examined using a battery of clinical, electrophysiological, immunological, and morphological methods. Myelin-immunized rats developed the characteristic features of EAN. P2-sensitized rats developed a similar but much less intense disorder. When rats were sensitized with P2 in the presence of phosphatidyl serine, however, they developed radiculoneuropathy that was indistinguishable from myelin-induced EAN. Inoculation with phosphatidyl serine plus complete Freund's adjuvant or complete Freund's adjuvant alone had no detectable effect on peripheral nerves. These studies demonstrate that sensitization of rats with a single myelin antigen, P2 protein, is sufficient to induce the clinical, electrophysiological, and neuropathological features of EAN.
髓磷脂P2蛋白被认为是全髓磷脂诱导的实验性变应性神经炎（EAN）中的主要抗原。我们通过用含有P2、P2加磷脂酰丝氨酸或含有等量P2的全髓磷脂的完全弗氏佐剂（CFA）致敏Lewis大鼠，研究了P2的神经原性特性。使用一系列临床、电生理、免疫学和形态学方法对动物进行检查。髓磷脂免疫的大鼠出现了EAN的特征性表现。P2致敏的大鼠出现了类似但程度轻得多的病症。然而，当大鼠在磷脂酰丝氨酸存在的情况下用P2致敏时，它们出现了与髓磷脂诱导的EAN无法区分的神经根神经病。接种磷脂酰丝氨酸加完全弗氏佐剂或单独接种完全弗氏佐剂对周围神经没有可检测到的影响。这些研究表明，用单一髓磷脂抗原P2蛋白致敏大鼠足以诱导EAN的临床、电生理和神经病理学特征。
