Hilmy A M, Kandeel K M, Selim N M
Arch Geschwulstforsch. 1984;54(6):475-82.
Pancreatic cancer was induced in male Sprague-Dawley rats by 7,12-dimethylbenz(a)anthracene. Pancreatic amylase has been purified from normal as well as treated rats after 2, 3 and 4 months of exposure to the carcinogen. The level of pancreatic amylase in rats with pancreatic carcinoma was significantly decreased (L.S.D.-5.25). Purified enzyme was then subjected to disc gel electrophoresis. Both normal and treated rats gave the same electrophoretic pattern (three bands: two major, and one minor). Therefore, there was no isoenzyme component in pancreatic extracts of rats bearing pancreatic cancer that could be held to be peculiar for pancreatic cancer. Histological findings showed a decrease in zymogen content together with its total absence in some areas of malignant cells. The data obtained suggested that the original carcinogenic events were associated with a decrease in amylase initial activity, and did not involve alteration in gene expression related to amylase biosynthesis.
通过7,12-二甲基苯并(a)蒽诱导雄性Sprague-Dawley大鼠患胰腺癌。在暴露于致癌物2、3和4个月后，从正常大鼠以及经处理的大鼠中纯化出胰腺淀粉酶。患有胰腺癌的大鼠胰腺淀粉酶水平显著降低（最小显著差异为-5.25）。然后将纯化的酶进行圆盘凝胶电泳。正常大鼠和经处理的大鼠呈现相同的电泳图谱（三条带：两条主要带和一条次要带）。因此，患有胰腺癌的大鼠胰腺提取物中不存在可被认为是胰腺癌特有的同工酶成分。组织学检查结果显示，酶原含量减少，并且在恶性细胞的某些区域完全不存在。所获得的数据表明，最初的致癌事件与淀粉酶初始活性降低有关，并且不涉及与淀粉酶生物合成相关的基因表达改变。
