编码小鼠α样胚胎珠蛋白链x的cDNA的分子克隆及序列分析

Molecular cloning and sequence analysis of a cDNA coding for the mouse alpha-like embryonic globin chain x.

作者信息

Farace M G, Hill A, Tripodi M, Padgett R W, Raschella G, Gambari R, Fantoni A, Hutchison C A, Edgell M H

出版信息

Gene. 1984 Nov;31(1-3):241-5. doi: 10.1016/0378-1119(84)90215-4.

Abstract

Cytoplasmic poly(A)+mRNA from 12-day mouse-yolk-sac erythroid cells has been used to prepare a cDNA library in the plasmid pBR322. One clone containing sequences coding for the alpha-like embryonic globin chain x, pHE52, has been identified by hybrid selection and in vitro translation of the complementary mRNA. The nucleotide sequence of pHE52 confirms that it codes for an embryonic alpha-like globin chain. The insert sequence is 316 nucleotides long, contains the codons corresponding to amino acid residues 43-141, and extends into the 3' untranslated region. An analysis of the nucleotide sequence of pHE52 and the other known alpha globins suggests that the adult-embryonic divergence began approx. 400 million years ago reflecting a difference in the evolutionary history of the alpha- and beta-globin gene complexes.

摘要

来自12日龄小鼠卵黄囊红细胞的细胞质多聚腺苷酸（poly(A)）+mRNA已被用于在质粒pBR322中制备cDNA文库。通过杂交筛选和互补mRNA的体外翻译，鉴定出一个包含编码α样胚胎珠蛋白链x（pHE52）序列的克隆。pHE52的核苷酸序列证实它编码一种胚胎α样珠蛋白链。插入序列长316个核苷酸，包含对应于氨基酸残基43 - 141的密码子，并延伸至3'非翻译区。对pHE52和其他已知α珠蛋白的核苷酸序列分析表明，成人 - 胚胎的分化大约始于4亿年前，这反映了α和β珠蛋白基因复合体进化历史的差异。