成年鸡β1珠蛋白cDNA的分子克隆与序列分析
Molecular cloning and sequence analysis of adult chicken betal globin cDNA.
作者信息
Richards R I, Shine J, Ullrich A, Wells J R, Goodman H M
出版信息
Nucleic Acids Res. 1979 Nov 10;7(5):1137-46. doi: 10.1093/nar/7.5.1137.
The molecular cloning and nucleotide sequence analysis of adult chicken beta globin mRNA is reported. DNA sequences derived from in vitro transcrption of globin mRNA were purified and amplified as recombinant DNA using the plasmid pBR322. Sequence analysis of several clones coding for beta globin strongly suggests that transcription errors may be generated near the 5' end of transcripts in vitro by reverse transcription. The complete sequence of the longest beta globin insert containing 51 bases of the 5' untranslated region as well as the complete coding and 3' untranslated regions has been determined.
本文报道了成年鸡β珠蛋白mRNA的分子克隆及核苷酸序列分析。从珠蛋白mRNA的体外转录物中获得的DNA序列，利用质粒pBR322进行纯化并作为重组DNA进行扩增。对几个编码β珠蛋白的克隆进行的序列分析强烈表明，体外逆转录过程中可能在转录物的5′端附近产生转录错误。已确定了最长的β珠蛋白插入片段的完整序列，该片段包含5′非翻译区的51个碱基以及完整的编码区和3′非翻译区。
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