一种生成对1,25 - 二羟基麦角钙化醇和1,25 - 二羟基胆钙化醇具有特异性抗体的简单方法。

A simple method for generation of antibodies with specificity for 1,25-dihydroxyergocalciferol and 1,25-dihydroxycholecalciferol.

作者信息

Clemens T L, Henderson S, Meng X, Baggiolini E G, Uskokovic M R, Holick S A, Holick M F

出版信息

Steroids. 1983 Nov;42(5):503-9. doi: 10.1016/0039-128x(83)90114-9.

Abstract

A simple method for production of antisera with high affinity and selectivity for 1 alpha, 25-dihydroxyergocalciferol and 1 alpha, 25-dihydroxychole-calciferol is described. 1 alpha-Hydroxy-25,26,27-trisnorcholecalciferol-24-oic acid was coupled directly to bovine serum albumin. Rabbits immunized with this conjugate rapidly produced antibodies that bound 3H-1 alpha,-25-dihydroxycholecalciferol with high affinity and demonstrated nearly equal reactivity with 1 alpha, 25-dihydroxyergocalciferol and poor reactivity with 25-hydroxycholecalciferol; 24,25-dihydroxycholecalciferol; 25,26-dihydroxycholecalciferol; and 1 beta,25-dihydroxycholecalciferol. The use of one of these antisera has led to the development of a specific assay for 1 alpha,25-dihydroxyergocalciferol and 1 alpha,25-dihydroxycholecalciferol in human serum.

摘要

描述了一种生产对1α,25 - 二羟基麦角钙化醇和1α,25 - 二羟基胆钙化醇具有高亲和力和选择性抗血清的简单方法。1α - 羟基 - 25,26,27 - 三降胆钙化醇 - 24 - 酸直接与牛血清白蛋白偶联。用这种偶联物免疫的兔子迅速产生抗体，该抗体以高亲和力结合3H - 1α,-25 - 二羟基胆钙化醇，对1α,25 - 二羟基麦角钙化醇表现出几乎相同的反应性，而对25 - 羟基胆钙化醇、24,25 - 二羟基胆钙化醇、25,26 - 二羟基胆钙化醇和1β,25 - 二羟基胆钙化醇反应性较差。使用其中一种抗血清已开发出一种用于检测人血清中1α,25 - 二羟基麦角钙化醇和1α,25 - 二羟基胆钙化醇的特异性测定方法。