一种针对1,25 - 二羟胆钙化醇的放射免疫测定法。
A radioimmunoassay for 1,25-dihydroxycholecalciferol.
作者信息
Clemens T L, Hendy G N, Graham R F, Baggiolini E G, Uskokovic M R, O'Riordan J L
出版信息
Clin Sci Mol Med. 1978 Mar;54(3):329-32. doi: 10.1042/cs0540329.
PMID:630809
Abstract
- 1alpha,25-Dihydroxy-25-hemisuccinate cholecalciferol has been synthesized and conjugated to bovine serum albumin. 2. This conjugate is immunogenic; when injected into rabbits antibodies of high affinity for 1alpha,25-dihydroxycholecalciferol were obtained. 3. Vitamin D metabolites lacking the 1alpha-hydroxy group were of lower cross-reactivity with the antibodies. 4. By using these antibodies and 1alpha,25-[23,24-3H]dihydroxycholecalciferol as tracer a sensitive radioimmunoassay has been developed capable of detecting 20 pg of 1alpha,25-dihydroxycholecalciferol.
摘要
- 已合成1α,25 - 二羟基 - 25 - 半琥珀酸胆钙化醇，并将其与牛血清白蛋白偶联。2. 这种偶联物具有免疫原性；注入兔子体内后，可获得对1α,25 - 二羟基胆钙化醇具有高亲和力的抗体。3. 缺乏1α - 羟基的维生素D代谢产物与这些抗体的交叉反应性较低。4. 通过使用这些抗体以及1α,25 - [23,24 - ³H]二羟基胆钙化醇作为示踪剂，已开发出一种灵敏的放射免疫分析法，能够检测到20皮克的1α,25 - 二羟基胆钙化醇。
Zotero 插件