Peters L J, McBride W H, Mason K A, Hunter N, Basíc I, Milas L
Dev Biol Stand. 1977;38:297-300.
We have investigated whether peritoneal exudate cells (PEC) from C. parvum (CP) treated (C3Hf/Bu mice could transfer in vivo the resistance against a syngeneic fibrosarcoma (FSa). Inhibition of tumour development and prolongation of survival of recipients were observed when CP-activated PEC were admixed with FSa cells before their intraperitoneal (ip) or subcutaneous (sc) injections into normal mice. The antitumour activity increased with the increase of the ratio of effector to target cells. Heat killed CP-PEC were unable to transfer the resistance. Also, pretreatment of recipients with 600 rads whole body irradiation (WBI) substantially reduced the efficacy of CP-PEC. Reconstitution of WBI mice with mixed normal spleen and lymph node cells, or spleen cells alone, or bone marrow cells did not restore the antitumor activity of transferred CP-PEC. In fact, reconstituted mice showed a further reduction of transferred antitumor resistance. CP-PEC activity was also inhibited in sc transfer experiments when normal PEC, spleen cells, T-cells or even fetal fibroblasts were admixed with tumor cells and CP-PEC. Possible reasons for the failure of WBI recipients to be fully protected by transferred CP-PEC are discussed.
我们研究了来自经微小隐孢子虫（CP）处理的（C3Hf/Bu小鼠）的腹腔渗出细胞（PEC）是否能在体内传递对同基因纤维肉瘤（FSa）的抗性。当CP激活的PEC在腹腔内（ip）或皮下（sc）注射到正常小鼠体内之前与FSa细胞混合时，观察到肿瘤发展受到抑制且受体存活时间延长。抗肿瘤活性随着效应细胞与靶细胞比例的增加而增强。热灭活的CP-PEC无法传递抗性。此外，用600拉德全身照射（WBI）预处理受体可显著降低CP-PEC的功效。用混合的正常脾细胞和淋巴结细胞、单独的脾细胞或骨髓细胞重建经WBI处理的小鼠，并未恢复转移的CP-PEC的抗肿瘤活性。事实上，重建后的小鼠转移的抗肿瘤抗性进一步降低。在皮下转移实验中，当正常PEC、脾细胞、T细胞甚至胎儿成纤维细胞与肿瘤细胞和CP-PEC混合时，CP-PEC活性也受到抑制。文中讨论了经WBI处理的受体未能被转移的CP-PEC完全保护的可能原因。
