蛋白质和核酸在缺乏共价交联的丙烯酰胺-琼脂糖凝胶上的电泳。

Electrophoresis of proteins and nucleic acids on acrylamide-agarose gels lacking covalent crosslinking.

作者信息

Horowitz P M, Lee J C, Williams G A, Williams R F, Barnes L D

出版信息

Anal Biochem. 1984 Dec;143(2):333-40. doi: 10.1016/0003-2697(84)90672-9.

Abstract

The preparation of acrylamide-agarose gels lacking covalent crosslinking with methylenebisacrylamide is described. These hybrid gels melt at 85 degrees C and, consequently, allow quantitative analysis of tritium-labeled protein after electrophoresis. Recovery of tritium-labeled ribonucleic acids extracted from hybrid gels is 20 to 25% greater than from standard acrylamide-methylenebisacrylamide gels. Standard curves of electrophoretic mobilities as a function of molecular weights of dissociated proteins and ribonucleic acids are compared for acrylamide-agarose gels and acrylamide-methylenebisacrylamide gels.

摘要

本文描述了不含与亚甲基双丙烯酰胺共价交联的丙烯酰胺-琼脂糖凝胶的制备方法。这些混合凝胶在85℃时熔化，因此可在电泳后对氚标记的蛋白质进行定量分析。从混合凝胶中提取的氚标记核糖核酸的回收率比从标准丙烯酰胺-亚甲基双丙烯酰胺凝胶中提取的高20%至25%。比较了丙烯酰胺-琼脂糖凝胶和丙烯酰胺-亚甲基双丙烯酰胺凝胶的电泳迁移率标准曲线，该曲线是解离蛋白质和核糖核酸分子量的函数。