用[U-14C]葡萄糖测定成年油橄榄实蝇（Gmelin）（双翅目实蝇科）中的氨基酸合成。

Amino-acid synthesis in adult Dacus oleae (Gmelin) (Diptera Tephritidae) determined with [U-14C] glucose.

作者信息

Tsiropoulos G J

出版信息

Arch Int Physiol Biochim. 1984 Nov;92(4):313-6. doi: 10.3109/13813458409071172.

Abstract

The ability of adult Dacus oleae for amino-acid synthesis from [U-14C] glucose was investigated. The relatively high specific activity radiometric measurements indicated that both sexes were able to synthesize the amino acids: alanine, aspartic acid, cystine, glutamic acid, glycine, hydroxyproline, proline and tyrosine; therefore, these amino acids are considered as nutritionally dispensable for D. oleae. On the other hand, the amino acids: arginine, histidine, leucine, isoleucine, lysine, methionine, phenylalanine, serine, threonine, and valine, showed a very low specific activity and therefore are considered as nutritionally indispensable. It was not possible to conclude about tryptophane, since the acid hydrolysis destroyed this amino acid.

摘要

研究了成年油橄榄实蝇利用[U-14C]葡萄糖合成氨基酸的能力。相对较高的比活度放射性测量表明，两性都能够合成丙氨酸、天冬氨酸、胱氨酸、谷氨酸、甘氨酸、羟脯氨酸、脯氨酸和酪氨酸；因此，这些氨基酸被认为对油橄榄实蝇在营养上是可替代的。另一方面，精氨酸、组氨酸、亮氨酸、异亮氨酸、赖氨酸、蛋氨酸、苯丙氨酸、丝氨酸、苏氨酸和缬氨酸的比活度非常低，因此被认为在营养上是必需的。由于酸水解会破坏色氨酸，所以无法对其作出结论。