Hojman-Montes de Oca F, Lasneret J, Dianoux L, Canivet M, Ravicovitch-Ravier R, Périès J
Biol Cell. 1984;52(3):199-204. doi: 10.1111/j.1768-322x.1985.tb00337.x.
The methylation state of Intracisternal A Particle (IAP) genes in mouse teratocarcinoma cell lines has been investigated as an approach to study the regulation of the expression of these particles. Treatment of the cells with the methylation inhibitor 5-azacytidine induces the production of the particles in all the cells; the induction is particularly striking in an embryonal carcinoma cell line which is normally devoid of IAPs. The induction is accompanied by decrease in DNA methylation as demonstrated by using the methylation sensitive isoschizomer enzymes MspI and HpaII. Hypomethylation of the IAP genes correlates with accumulation of IAP, specific polyadenylated RNA reinforcing the hypothesis that methylation plays an important role in the control of IAP expression.
对小鼠畸胎瘤细胞系中内池A颗粒（IAP）基因的甲基化状态进行了研究，以此作为研究这些颗粒表达调控的一种方法。用甲基化抑制剂5-氮杂胞苷处理细胞可诱导所有细胞产生这些颗粒；在通常不含IAP的胚胎癌细胞系中，这种诱导尤为显著。如使用甲基化敏感的同裂酶MspI和HpaII所证明的，诱导伴随着DNA甲基化的减少。IAP基因的低甲基化与IAP的积累相关，特定的多聚腺苷酸化RNA强化了甲基化在IAP表达控制中起重要作用这一假说。
