Serrou B, Domas J
Dev Biol Stand. 1977;38:515-21.
We treated one hundred patients who had various high risk solid tumors (malignant melanomas, osteosarcomas and lung cancers) by immunostimulation alone or with a sequential and synchronized chemotherapy as a complement treatment. Institut Pasteur BCG (150 mg) was administered either by scarifications (10 X 10 of 5 cm each) or multiple puncture technique (Gun), or in the case of 12 patients, by intra-tumor injections. The following complications were observed: chills and high fever during 1 to 30 days after scarifications or gun technique. In some cases an allergic loco-regional cutaneous reaction was noted after the gun technique. Nevertheless these complications were well tolerated. However, severe reactions were observed after the intra-tumor injections: malaise, chills, sweating, hyperthermia, nausea, vomiting and changes in blood pressure. In 1 case a prolonged high fever (3 weeks) was offset only by the use of corticosteroids. In another case the patient developed hepatitis. A percutaneous liver biopsy revealed noncaseating granulomas and the presence of acid fast organisms in the liver (by means of staining by auramine and observation by fluorescence). In this patient BCG has been replaced by Corynebacterium parvum (2 X 2 mg a week). This type of adjuvant was used in 2 patients and produced the same complications as the BCG. We believe that caution must be exercised in the use of such intra-tumoral treatments. BCG must be given in the hospital and patients must receive antihistaminic preparation before and after immunostimulation.
我们对100例患有各种高危实体瘤（恶性黑色素瘤、骨肉瘤和肺癌）的患者进行了单独免疫刺激或联合序贯同步化疗作为补充治疗。巴斯德研究所卡介苗（150毫克）通过划痕法（每次5厘米，10×10）、多点穿刺技术（喷枪）给药，12例患者通过瘤内注射给药。观察到以下并发症：划痕法或喷枪技术后1至30天内出现寒战和高热。在某些情况下，喷枪技术后出现局部过敏皮肤反应。然而，这些并发症耐受性良好。然而，瘤内注射后观察到严重反应：不适、寒战、出汗、体温过高、恶心、呕吐和血压变化。1例患者持续高热（3周），仅通过使用皮质类固醇才得以缓解。另一例患者出现肝炎。经皮肝活检显示非干酪样肉芽肿，肝脏中存在抗酸菌（通过金胺染色和荧光观察）。在该患者中，卡介苗已被短小棒状杆菌（每周2×2毫克）取代。这种佐剂用于2例患者，产生了与卡介苗相同的并发症。我们认为，在使用这种瘤内治疗时必须谨慎。卡介苗必须在医院给药，患者在免疫刺激前后必须接受抗组胺制剂治疗。
