Dykes P W, Trejdosiewicz L K
Dev Biol Stand. 1977;38:547-52.
In a preliminary and toxicological study, eight patients with carcinoma of the stomach were treated by Corynebacterium parvum immunotherapy prior to surgical excision. Killed C. parvum suspension was injected at multiple sites directly into the tumour mass with the aid of fibre-optic gastroscope at doses of 1.5 mg, 4 mg or 8 mg. Each patient received two injections of the same dose with an interval of 14 days. The second injections were made 14 to 18 days prior to surgery. No chemotherapy was administered during this treatment. Patients were monitored clinically for signs of toxicity. Immunological parameters were assessed on peripheral blood samples taken immediately before each injection and prior to surgery. Antibody titre to C. parvum was estimated by direct agglutination, while cell-mediated responses to C. parvum and 3M KCl tumour extract and phytohaemagglutinin (PHA) were assessed by lymphocyte transformation. Serum carcinoembryonic antigen (CEA) was measured by radio-immune assay. Tumours removed at surgery were examined histologically for the presence of invading immunocytes. These preliminary data will be discussed with respect to the dose of C. parvum administered.
在一项初步毒理学研究中，8例胃癌患者在手术切除前接受了短小棒状杆菌免疫疗法治疗。借助纤维光学胃镜，将灭活的短小棒状杆菌悬液以1.5毫克、4毫克或8毫克的剂量在多个部位直接注入肿瘤块。每位患者接受两次相同剂量的注射，间隔14天。第二次注射在手术前14至18天进行。在此治疗期间未给予化疗。对患者进行临床监测以观察毒性迹象。在每次注射前及手术前采集外周血样本评估免疫参数。通过直接凝集法估计对短小棒状杆菌的抗体滴度，而通过淋巴细胞转化评估对短小棒状杆菌、3M氯化钾肿瘤提取物和植物血凝素（PHA）的细胞介导反应。通过放射免疫测定法测量血清癌胚抗原（CEA）。对手术切除的肿瘤进行组织学检查以确定是否存在浸润性免疫细胞。将结合所给予的短小棒状杆菌剂量对这些初步数据进行讨论。
