Roumiantzeff M, Colombet G
Dev Biol Stand. 1977;38:65-72.
A skin-testing antigen produced from C. parvum has been developed for exploring cell-mediated immunity and specially C. parvum specific cell-mediated immunity by delayed cutaneous hypersensitivity (DCH) reaction. The DCH antigen and the techniques of intradermal injection and multiple puncture are described. DCH reactions are carried out in C. parvum specifically sensitized guinea pigs: sensitization procedure and adjuvant (IFA and CFA) effects are reported. Measures of DCH reactions for different antigen doses at various times (5-24-48 h) are reported; classical Mantoux and multiple puncture reactions are compared. The specificity of these DCH reactions are explored by comparing C. parvum antigen and tuberculin reactions in corynebacterium and mycobacterium sensitized guinea pigs.
已开发出一种由微小隐孢子虫产生的皮肤测试抗原，用于通过迟发型皮肤超敏反应（DCH）来探索细胞介导的免疫，特别是微小隐孢子虫特异性细胞介导的免疫。文中描述了DCH抗原以及皮内注射和多点穿刺技术。在微小隐孢子虫特异性致敏的豚鼠中进行DCH反应：报告了致敏程序和佐剂（不完全弗氏佐剂和完全弗氏佐剂）的作用。报告了在不同时间（5 - 24 - 48小时）针对不同抗原剂量的DCH反应测量方法；比较了经典的结核菌素皮试反应和多点穿刺反应。通过比较微小隐孢子虫抗原与在棒状杆菌和分枝杆菌致敏的豚鼠中的结核菌素反应，来探索这些DCH反应的特异性。
