Fujita S C, Obata K
Neurosci Res. 1984 Apr;1(2):131-48. doi: 10.1016/s0168-0102(84)80010-3.
To explore the extent and significance of chemical diversity of neurons, monoclonal antibodies were generated that bind to the chick embryonic central nervous system with varying degrees of regional specificity. Mice were immunized against a homogenate of optic nerves from 8-day chick embryos. Antibody was screened by an indirect fluorescence immunohistochemical method using frozen sections of embryonic neural tissues. From 3 fusion experiments, 58 lines of hybrydoma were cloned. Twenty-three monoclonal antibodies were studied in detail, and here reported, for their characteristic staining patterns in the spinal cord of 6-day embryos. The majority of the antibodies bound preferentially to different subregions of spinal funiculi, in which glial elements are still undeveloped. At least 6 subregions could be distinguished in the funiculi, probably corresponding to the developing spinal tracts. For 8 of the antibodies, immunoreactive polypeptides were identified in an electrophoretic analysis.
为了探究神经元化学多样性的程度及其意义，制备了与鸡胚中枢神经系统具有不同程度区域特异性结合的单克隆抗体。用8日龄鸡胚的视神经匀浆免疫小鼠。使用胚胎神经组织的冰冻切片，通过间接荧光免疫组织化学方法筛选抗体。从3次融合实验中，克隆了58株杂交瘤细胞系。对23种单克隆抗体进行了详细研究，并在此报告它们在6日龄胚胎脊髓中的特征性染色模式。大多数抗体优先结合脊髓白质的不同亚区域，其中神经胶质成分仍未发育。在白质中至少可区分出6个亚区域，可能对应于正在发育的脊髓束。对于其中8种抗体，在电泳分析中鉴定出了免疫反应性多肽。
