Hybridization analysis of specific RNAs by electrophoretic separation in agarose gels and transfer to diazobenzyloxymethyl paper.
作者信息
Alwine J C
机构信息
Department of Microbiology, School of Medicine, University of Pennsylvania, Philadelphia 19104.
出版信息
Gene Amplif Anal. 1981;2:565-72.
PMID:6086041
Abstract
摘要
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Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.通过转移至重氮苄氧基甲基纸并与DNA探针杂交来检测琼脂糖凝胶中特定RNA的方法。
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5350-4. doi: 10.1073/pnas.74.12.5350.
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Transfer of small DNA fragments from polyacrylamide gels to diazobenzyloxymethyl-paper and detection by hybridization with DNA probes.将小DNA片段从聚丙烯酰胺凝胶转移至重氮苄氧基甲基纸，并通过与DNA探针杂交进行检测。
Biochem Biophys Res Commun. 1978 Dec 14;85(3):1104-12. doi: 10.1016/0006-291x(78)90656-3.
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Electrophoretic transfer of DNA, RNA and protein onto diazobenzyloxymethyl (DBM) - paper.DNA、RNA和蛋白质向重氮苄氧基甲基（DBM）纸的电泳转移。
Nucleic Acids Res. 1980 Jan 25;8(2):299-317. doi: 10.1093/nar/8.2.299.
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Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paper.通过凝胶分级分离并转移至重氮苄氧基甲基纸上检测特定RNA或DNA特定片段。
Methods Enzymol. 1979;68:220-42. doi: 10.1016/0076-6879(79)68017-5.
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Direct in-gel hybridization, without blotting, using nick-translated cloned DNA probe.使用缺口平移克隆DNA探针进行直接凝胶内杂交，无需印迹。
Biotechniques. 1991 Dec;11(6):718, 720, 722.
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Electrophoretic transfer of DNA, RNA, and protein onto diazobenzyloxymethyl paper.DNA、RNA和蛋白质向重氮苄氧基甲基纸的电泳转移。
Methods Enzymol. 1983;100:326-33. doi: 10.1016/0076-6879(83)00065-8.
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Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.通过使用硫酸葡聚糖将大的DNA片段从琼脂糖凝胶高效转移至重氮苄氧基甲基纸并进行快速杂交。
Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7. doi: 10.1073/pnas.76.8.3683.
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Direct in-gel hybridization of digoxigenin-labelled non-radioactive probes.地高辛标记的非放射性探针的直接凝胶内杂交。
Mol Cell Probes. 1999 Jun;13(3):233-7. doi: 10.1006/mcpr.1999.0241.
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A rapid method for detecting specific RNA transcripts by hybridization to DNA probes in solution.
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