Chae K, Albro P W, Luster M I, McKinney J D
Int J Environ Anal Chem. 1984;17(3-4):267-74. doi: 10.1080/03067318408076978.
A relatively simple assay method for the putative cytosolic 'receptor' that binds 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and related compounds is described. The assay is based on specific binding of [125I]dioxin to cytosol 'receptor' protein. Saturation is ensured by competition experiments in which unlabeled TCDD and other competitors displace the radiolabeled ligand from specific binding sites. This assay has been applied to estimation of levels of 'receptor' in cytosol.
本文描述了一种相对简单的检测方法，用于检测与2,3,7,8-四氯二苯并对二恶英（TCDD）及相关化合物结合的假定胞质“受体”。该检测基于[125I]二恶英与胞质“受体”蛋白的特异性结合。通过竞争实验确保饱和，在竞争实验中，未标记的TCDD和其他竞争者将放射性标记的配体从特异性结合位点上置换下来。该检测方法已应用于胞质中“受体”水平的测定。
