Tsuda H, Takeshige K, Shibata Y, Minakami S
J Biochem. 1984 May;95(5):1237-45. doi: 10.1093/oxfordjournals.jbchem.a134727.
Human colostral macrophages stimulated by opsonized zymosan or phorbol myristate acetate (PMA) released superoxide anions (O2-) and hydrogen peroxide (H2O2) with activities comparable to those of monocytes and about one-fourth of those of polymorphonuclear leukocytes (PMNL) of blood. The O2- -forming oxidase in the macrophages stimulated by PMA was dependent on NADPH as an electron donor with an apparent Km value for NADPH of 27.6 +/- 4.0 microM, which is comparable to those obtained for the stimulated monocytes and PMNL of blood. The Vmax was 1.86 +/- 0.33 nmol O2/min/10(6) cells, which is essentially the same as that of monocytes and about half of that of PMNL. p-Chloromercuribenzoate or cetyltrimethylammonium bromide completely inhibited oxidases of all three types of phagocytes. A b-type cytochrome was identified in the macrophages but the concentrations in the macrophages and monocytes were less than half of that in PMNL. These results suggest that the differences in the O2- -forming activities of the three types of phagocytes are quantitative rather than qualitative. The macrophages and monocytes showed very low activities of myeloperoxidase [EC 1.11.1.7] in contrast to PMNL. The activity of beta-glucuronidase [EC 3.2.1.31] in the macrophages was much higher than those of the monocytes and PMNL, but little difference was observed in the activities of lysozyme [EC 3.2.1.17], catalase [EC 1.11.1.6] and superoxide dismutase [EC 1.15.1.1] among the three types of phagocytes examined. Electron micrographs of the macrophages showed little increase of vacuoles upon exposure to PMA, in contrast to the cases of monocytes and PMNL.
经调理酵母聚糖或佛波酯（PMA）刺激的人初乳巨噬细胞释放超氧阴离子（O2-）和过氧化氢（H2O2），其活性与单核细胞相当，约为血液中多形核白细胞（PMNL）活性的四分之一。PMA刺激的巨噬细胞中形成O2-的氧化酶依赖于NADPH作为电子供体，NADPH的表观Km值为27.6±4.0μM，这与受刺激的单核细胞和血液中的PMNL所获得的值相当。Vmax为1.86±0.33 nmol O2/分钟/10(6)个细胞，这与单核细胞基本相同，约为PMNL的一半。对氯汞苯甲酸或十六烷基三甲基溴化铵完全抑制了所有三种类型吞噬细胞的氧化酶。在巨噬细胞中鉴定出一种b型细胞色素，但巨噬细胞和单核细胞中的浓度不到PMNL中的一半。这些结果表明，三种类型吞噬细胞形成O2-的活性差异是定量的而非定性的。与PMNL相比，巨噬细胞和单核细胞显示出非常低的髓过氧化物酶[EC 1.11.1.7]活性。巨噬细胞中β-葡萄糖醛酸酶[EC 3.2.1.31]的活性远高于单核细胞和PMNL，但在所检测的三种类型吞噬细胞中，溶菌酶[EC 3.2.1.17]、过氧化氢酶[EC 1.11.1.6]和超氧化物歧化酶[EC 1.15.1.1]的活性几乎没有差异。与单核细胞和PMNL的情况相比，巨噬细胞的电子显微镜照片显示，暴露于PMA后液泡增加很少。
