Singh S P, Chhabra R, Srivastava V M
Division of Biochemistry, Central Drug Research Institute, Lucknow, India.
Experientia. 1992 Oct 15;48(10):994-6. doi: 10.1007/BF01919150.
Intraperitoneal administration of tuftsin-M [Thr-Lys-Pro-Arg-NH-(CH2)2-NH-CO-C15H31] to Balb/C mice has been shown to induce a respiratory burst in the peritoneal exudate cells. The macrophages exhibited enhanced levels of O2-, H2O2, NADPH oxidase and myeloperoxidase, but the activities of superoxide dismutase, catalase and glutathione peroxidase remained virtually unchanged. The magnitude of the oxidative burst depended directly on the dose of tuftsin-M; higher activity was observed at higher doses of the peptide. Tuftsin-M enhanced the generation of both O2- and H2O2 under in vitro conditions, as did phorbol myristate acetate. These results suggest that tuftsin-M could enhance non-specific defence against infections by activating the macrophages.
已证明，向Balb/C小鼠腹腔注射促吞噬素-M [苏氨酸-赖氨酸-脯氨酸-精氨酸-NH-(CH2)2-NH-CO-C15H31] 可诱导腹腔渗出细胞产生呼吸爆发。巨噬细胞中O2-、H2O2、NADPH氧化酶和髓过氧化物酶水平升高，但超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的活性基本保持不变。氧化爆发的程度直接取决于促吞噬素-M的剂量；肽剂量越高，活性越高。在体外条件下，促吞噬素-M与佛波酯一样，均可增强O2-和H2O2的生成。这些结果表明，促吞噬素-M可通过激活巨噬细胞来增强对感染的非特异性防御。
