啤酒花矮化类病毒的双链互补脱氧核糖核酸具有感染性。
Double-stranded cDNAs of hop stunt viroid are infectious.
作者信息
Meshi T, Ishikawa M, Ohno T, Okada Y, Sano T, Ueda I, Shikata E
出版信息
J Biochem. 1984 May;95(5):1521-4. doi: 10.1093/oxfordjournals.jbchem.a134761.
Abstract
Restriction fragments composed of only hop stunt viroid (HSV) cDNA were prepared from recombinant clone pHS-P4P, which carries four tandemly repeated HSV cDNAs, and inoculated into cucumber cotyledons. The results showed that double-stranded cDNAs consisting of 1 to 3 units of HSV sequences were infectious. In cucumber plants inoculated with these double-stranded cDNAs, infectious RNA molecules indistinguishable from authentic HSV were propagated.
摘要
从携带四个串联重复的啤酒花矮化类病毒（HSV）cDNA的重组克隆pHS - P4P中制备仅由HSV cDNA组成的限制性片段，并将其接种到黄瓜子叶中。结果表明，由1至3个HSV序列单元组成的双链cDNA具有感染性。在用这些双链cDNA接种的黄瓜植株中，传播了与正宗HSV无法区分的感染性RNA分子。
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