Orchansky P, Novick D, Fischer D G, Rubinstein M
J Interferon Res. 1984 Spring;4(2):275-82. doi: 10.1089/jir.1984.4.275.
Binding site competition studies of 125I-interferon (IFN)-gamma were performed with homogeneous preparations of IFNs alpha 2, beta, and gamma. It was found that only IFN-gamma could compete for the specific binding site of 125I-IFN-gamma in both WISH and FS11 cells. Thus, a clear distinction between the receptor of IFN- alpha and beta on one hand, and the receptor of IFN-gamma on the other hand, was obtained. This result is unambiguous because all IFN preparations which were used were purified to homogeneity by specific monoclonal antibody immunoaffinity chromatography. We propose to assign the names "Type I" for the receptor of IFN-alpha and IFN-beta, and "Type II" for the specific receptor of IFN-gamma.
利用α2、β和γ干扰素的均一制剂进行了125I-γ干扰素的结合位点竞争研究。结果发现，只有γ干扰素能够在WISH和FS11细胞中竞争125I-γ干扰素的特异性结合位点。因此，明确区分了α和β干扰素的受体与γ干扰素的受体。由于所有使用的干扰素制剂均通过特异性单克隆抗体免疫亲和层析纯化至均一性，所以这一结果是明确无误的。我们建议将α和β干扰素的受体命名为“Ⅰ型”，将γ干扰素的特异性受体命名为“Ⅱ型”。
