Pech M, Jaenichen H R, Pohlenz H D, Neumaier P S, Klobeck H G, Zachau H G
J Mol Biol. 1984 Jun 25;176(2):189-204. doi: 10.1016/0022-2836(84)90420-0.
An 80,000 base-pair region from the gene locus encoding the variable regions of the human immunoglobulins of the kappa type (V kappa genes) was cloned and analysed. The region comprises five V kappa sequences of subgroup I and one interspersed V kappa pseudogene of subgroup II. The six genes and pseudogenes are arranged at different distances but in the same orientation. The organization of the cluster can be explained by a series of amplification steps; the existence of a V kappa II pseudogene in a V kappa I gene cluster may have been the result of a transposition event; a final duplication step led to a second closely related copy of the cluster. From sequence data for altogether 16,000 base-pairs it appears that gene conversion-like events and subsequent selection contribute to both homogeneity and diversity of the V kappa repertoire.
对编码人κ型免疫球蛋白可变区（Vκ基因）的基因座的一个80,000个碱基对区域进行了克隆和分析。该区域包含I亚组的五个Vκ序列和一个散布于其中的II亚组Vκ假基因。这六个基因和假基因以不同距离排列，但方向相同。该基因簇的组织方式可通过一系列扩增步骤来解释；VκI基因簇中VκII假基因的存在可能是转座事件的结果；最后一次重复步骤导致了该基因簇的第二个密切相关的拷贝。从总共16,000个碱基对的序列数据来看，类似基因转换的事件及随后的选择作用对Vκ基因库的同质性和多样性均有贡献。
