Prasad C, Edwards R M
Neurochem Res. 1984 Apr;9(4):545-53. doi: 10.1007/BF00964381.
The specific binding of thyrotropin-releasing hormone (TRH) by 30,000 g pellet fraction was ubiquitously distributed throughout various rat brain regions including cerebellum. Although the cerebellum had the lowest apparent density of specific TRH binding sites found in any of the brain regions studied, it represented a single class of high affinity receptor (KD = 37.73 +/- 4.88 nM, Bmax = 156.0 +/- 5.7 fmol/mg protein, n = 4). Furthermore, the cerebellar synaptic plasma membrane fractions were richly endowed with TRH-binding, two other membrane fractions (light-synaptic plasma membrane and microsomal) exhibited high TRH-binding whereas nuclear, mitochondrial or myelin fractions were devoid of significant binding activity. These data show for the first time the existence of specific TRH-binding in cerebellum, and thus suggest that TRH may modulate cerebellar synaptic functions by acting through a specific high affinity-receptor.
促甲状腺激素释放激素（TRH）与30,000g沉淀组分的特异性结合在包括小脑在内的大鼠各个脑区中普遍分布。尽管在所有研究的脑区中，小脑的特异性TRH结合位点的表观密度最低，但它代表了一类单一的高亲和力受体（解离常数KD = 37.73±4.88 nM，最大结合容量Bmax = 156.0±5.7 fmol/mg蛋白质，n = 4）。此外，小脑突触质膜组分富含TRH结合，另外两个膜组分（轻突触质膜和微粒体）也表现出高TRH结合，而核、线粒体或髓鞘组分则没有明显的结合活性。这些数据首次表明小脑存在特异性TRH结合，因此提示TRH可能通过特异性高亲和力受体发挥作用来调节小脑突触功能。
