Pacheco M F, McKelvy J F, Woodward D J, Loudes C, Joseph-Bravo P, Krulich L, Griffin W S
Peptides. 1981 Fall;2(3):277-82. doi: 10.1016/s0196-9781(81)80119-2.
We determined the regional distribution and concentration of endogenous TRH in the rat cerebellum. Radioimmunoassay of endogenous TRH extracted and purified from five different regions of the rat cerebellum and whole hypothalamus showed that the cerebellar vermis contained 24 pg/mg, the hemispheres 74 pg/mg, the deep cerebellar nuclei 148 pg/mg, and the flocculo-nodular region 559 pg/mg of TRH. The highest concentration of TRH was in the cerebellar paraflocculi, which contained 786 pg/mg. The hypothalamic concentration of TRH was 465 pg/mg. Assay of the non-purified tissue fractions (crude extracts) resulted in lower TRH values in accordance with data previously reported by other authors. Bioassay analysis of TRH in purified fractions resulted in values similar to those obtained by radioimmunoassay. On the basis of these findings we hypothesize a functional role for TRH in the cerebellum.
我们测定了大鼠小脑中内源性促甲状腺激素释放激素（TRH）的区域分布和浓度。对从大鼠小脑五个不同区域及整个下丘脑提取并纯化的内源性TRH进行放射免疫分析，结果显示，小脑蚓部含TRH 24 pg/mg，小脑半球含74 pg/mg，小脑深部核团含148 pg/mg，绒球小结叶含559 pg/mg。TRH浓度最高的是小脑旁绒球，其含786 pg/mg。下丘脑TRH浓度为465 pg/mg。对未纯化的组织部分（粗提物）进行分析，得到的TRH值较低，这与其他作者先前报道的数据一致。对纯化部分的TRH进行生物测定分析，得到的值与放射免疫分析所得值相似。基于这些发现，我们推测TRH在小脑中具有功能性作用。
