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大肠杆菌DNA的rpoBC衰减子区域的野生型核苷酸序列及其对rifd18突变性质的影响。

The wild-type nucleotide sequence of the rpoBC-attenuator region of Escherichia coli DNA and its implications for the nature of the rifd18 mutation.

作者信息

Morgan B A, Kellett E, Hayward R S

出版信息

Nucleic Acids Res. 1984 Jul 11;12(13):5465-70. doi: 10.1093/nar/12.13.5465.

DOI:10.1093/nar/12.13.5465
PMID:6087288
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC318932/
Abstract

To investigate the possibility that the unusual dominant rifampicin-resistance characteristic of the rifd18 allele of E. coli rpoB is due to a secondary, regulatory mutation, we have determined the nucleotide sequence of a 1.1 Kbp wild-type DNA fragment, including the transcriptional attenuator and translational start-site of rpoB. We have also re-investigated the previously published sequences of this region in lambda rifd18 and lambda rifd47 DNA. Our results indicate that all three sequences are identical, and reveal some errors in the published data. We discuss the basis of dominance of rifd18.

摘要

为了研究大肠杆菌rpoB的rifd18等位基因不同寻常的显性利福平抗性特征是否源于继发性调控突变，我们测定了一段1.1 Kbp野生型DNA片段的核苷酸序列，包括rpoB的转录衰减子和翻译起始位点。我们还重新研究了此前发表的λrifd18和λrifd47 DNA中该区域的序列。我们的结果表明，所有这三个序列都是相同的，并揭示了已发表数据中的一些错误。我们讨论了rifd18显性的基础。

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1
The wild-type nucleotide sequence of the rpoBC-attenuator region of Escherichia coli DNA and its implications for the nature of the rifd18 mutation.大肠杆菌DNA的rpoBC衰减子区域的野生型核苷酸序列及其对rifd18突变性质的影响。
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本文引用的文献

1
Nucleotide sequence of the proximal portion of the RNA polymerase beta subunit gene of Escherichia coli.大肠杆菌RNA聚合酶β亚基基因近端部分的核苷酸序列。
Gene. 1980 Nov;11(3-4):367-73. doi: 10.1016/0378-1119(80)90076-1.
2
Characterization of promoter-cloning plasmids: analysis of operon structure in the rif region of Escherichia coli and isolation of an enhanced internal promoter mutant.启动子克隆质粒的特性分析：大肠杆菌rif区域操纵子结构的分析及增强型内部启动子突变体的分离
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Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.缓冲液梯度凝胶和35S标记辅助快速DNA序列测定。
Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963-5. doi: 10.1073/pnas.80.13.3963.
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RNA polymerase rifampicin resistance mutations in Escherichia coli: sequence changes and dominance.大肠杆菌中RNA聚合酶利福平抗性突变：序列变化与显性
Mol Gen Genet. 1983;190(2):344-8. doi: 10.1007/BF00330662.
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A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.一对用于选择双酶切限制片段任一条DNA链的新型M13载体。
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Nucleotide sequence at the end of the gene for the RNA polymerase beta' subunit (rpoC).RNA聚合酶β'亚基（rpoC）基因末端的核苷酸序列。
Nucleic Acids Res. 1981 Dec 21;9(24):6827-40. doi: 10.1093/nar/9.24.6827.
7
The primary structure of Escherichia coli RNA polymerase. Nucleotide sequence of the rpoB gene and amino-acid sequence of the beta-subunit.大肠杆菌RNA聚合酶的一级结构。rpoB基因的核苷酸序列和β亚基的氨基酸序列。
Eur J Biochem. 1981 Jun 1;116(3):621-9. doi: 10.1111/j.1432-1033.1981.tb05381.x.
8
Cloning of DNA of the rpoBC operon from the chromosome of Escherichia coli K12.从大肠杆菌K12染色体中克隆rpoBC操纵子的DNA。
Mol Gen Genet. 1980 Feb;177(3):527-33. doi: 10.1007/BF00271493.
9
Attenuation and processing of RNA from the rplJL--rpoBC transcription unit of Escherichia coli.大肠杆菌rplJL - rpoBC转录单元RNA的衰减与加工
Proc Natl Acad Sci U S A. 1980 Jun;77(6):3331-5. doi: 10.1073/pnas.77.6.3331.
10
Interaction of RNA polymerase mutations in haploid and merodiploid cells of Escherichia coli K-12.大肠杆菌K-12单倍体和部分二倍体细胞中RNA聚合酶突变的相互作用
Mol Gen Genet. 1971;110(2):118-33. doi: 10.1007/BF00332643.