大肠杆菌DNA的rpoBC衰减子区域的野生型核苷酸序列及其对rifd18突变性质的影响。
The wild-type nucleotide sequence of the rpoBC-attenuator region of Escherichia coli DNA and its implications for the nature of the rifd18 mutation.
作者信息
Morgan B A, Kellett E, Hayward R S
出版信息
Nucleic Acids Res. 1984 Jul 11;12(13):5465-70. doi: 10.1093/nar/12.13.5465.
Abstract
To investigate the possibility that the unusual dominant rifampicin-resistance characteristic of the rifd18 allele of E. coli rpoB is due to a secondary, regulatory mutation, we have determined the nucleotide sequence of a 1.1 Kbp wild-type DNA fragment, including the transcriptional attenuator and translational start-site of rpoB. We have also re-investigated the previously published sequences of this region in lambda rifd18 and lambda rifd47 DNA. Our results indicate that all three sequences are identical, and reveal some errors in the published data. We discuss the basis of dominance of rifd18.
摘要
为了研究大肠杆菌rpoB的rifd18等位基因不同寻常的显性利福平抗性特征是否源于继发性调控突变，我们测定了一段1.1 Kbp野生型DNA片段的核苷酸序列，包括rpoB的转录衰减子和翻译起始位点。我们还重新研究了此前发表的λrifd18和λrifd47 DNA中该区域的序列。我们的结果表明，所有这三个序列都是相同的，并揭示了已发表数据中的一些错误。我们讨论了rifd18显性的基础。
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2
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J Bacteriol. 1980 Dec;144(3):904-16. doi: 10.1128/jb.144.3.904-916.1980.
3
Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.缓冲液梯度凝胶和35S标记辅助快速DNA序列测定。
Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963-5. doi: 10.1073/pnas.80.13.3963.
4
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5
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6
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