Carlson J, Fuchs J A, Messing J
Proc Natl Acad Sci U S A. 1984 Jul;81(14):4294-7. doi: 10.1073/pnas.81.14.4294.
The nucleotide sequence of the Escherichia coli K-12 DNA comprising the operon for the structural genes of the subunits of ribonucleotide diphosphate reductase has been determined. The DNA sequenced maps at 48.5 minutes on the E. coli chromosome and includes a total length of 8557 nucleotides. An open reading frame between nucleotides 3506 and 5834, encoding a 776-amino acid polypeptide chain with a molecular weight of 87,532, has been identified as the nrdA gene. An open reading frame between nucleotides 6012 and 7139, encoding a 375-amino acid polypeptide with a molecular weight of 43,466, has been identified as the nrdB gene. The sequences reveal not only the primary structures for both subunits, but also some interesting aspects of potential regulatory sites.
已确定包含核糖核苷酸二磷酸还原酶亚基结构基因操纵子的大肠杆菌K-12 DNA的核苷酸序列。该DNA序列位于大肠杆菌染色体上48.5分钟处，全长8557个核苷酸。已鉴定出核苷酸3506至5834之间的一个开放阅读框，其编码一条分子量为87532的776个氨基酸的多肽链，该开放阅读框被确定为nrdA基因。已鉴定出核苷酸6012至7139之间的一个开放阅读框，其编码一条分子量为43466的375个氨基酸的多肽，该开放阅读框被确定为nrdB基因。这些序列不仅揭示了两个亚基的一级结构，还揭示了潜在调控位点的一些有趣方面。
