大肠杆菌核糖核苷二磷酸还原酶操纵子的一级结构。
Primary structure of the Escherichia coli ribonucleoside diphosphate reductase operon.
作者信息
Carlson J, Fuchs J A, Messing J
出版信息
Proc Natl Acad Sci U S A. 1984 Jul;81(14):4294-7. doi: 10.1073/pnas.81.14.4294.
Abstract
The nucleotide sequence of the Escherichia coli K-12 DNA comprising the operon for the structural genes of the subunits of ribonucleotide diphosphate reductase has been determined. The DNA sequenced maps at 48.5 minutes on the E. coli chromosome and includes a total length of 8557 nucleotides. An open reading frame between nucleotides 3506 and 5834, encoding a 776-amino acid polypeptide chain with a molecular weight of 87,532, has been identified as the nrdA gene. An open reading frame between nucleotides 6012 and 7139, encoding a 375-amino acid polypeptide with a molecular weight of 43,466, has been identified as the nrdB gene. The sequences reveal not only the primary structures for both subunits, but also some interesting aspects of potential regulatory sites.
摘要
已确定包含核糖核苷酸二磷酸还原酶亚基结构基因操纵子的大肠杆菌K-12 DNA的核苷酸序列。该DNA序列位于大肠杆菌染色体上48.5分钟处，全长8557个核苷酸。已鉴定出核苷酸3506至5834之间的一个开放阅读框，其编码一条分子量为87532的776个氨基酸的多肽链，该开放阅读框被确定为nrdA基因。已鉴定出核苷酸6012至7139之间的一个开放阅读框，其编码一条分子量为43466的375个氨基酸的多肽，该开放阅读框被确定为nrdB基因。这些序列不仅揭示了两个亚基的一级结构，还揭示了潜在调控位点的一些有趣方面。
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本文引用的文献
1
The making of strand-specific M13 probes.链特异性M13探针的制备。
Gene. 1982 Mar;17(3):271-7. doi: 10.1016/0378-1119(82)90143-3.
2
Construction and characterization of hybrid plasmids containing the Escherichia coli nrd region.含有大肠杆菌nrd区域的杂交质粒的构建与特性分析
J Bacteriol. 1980 Aug;143(2):561-8. doi: 10.1128/jb.143.2.561-568.1980.
3
Apple II computer software for DNA and protein sequence data.用于DNA和蛋白质序列数据的苹果II计算机软件。
DNA. 1983;2(1):31-5. doi: 10.1089/dna.1.1983.2.31.
4
Requirement of protein synthesis for the induction of ribonucleoside diphosphate reductase mRNA in Escherichia coli.蛋白质合成对大肠杆菌中核糖核苷二磷酸还原酶mRNA诱导的需求。
Mol Gen Genet. 1984;193(2):327-31. doi: 10.1007/BF00330689.
5
The primary structure of Escherichia coli glutaredoxin. Distant homology with thioredoxins in a superfamily of small proteins with a redox-active cystine disulfide/cysteine dithiol.大肠杆菌谷氧还蛋白的一级结构。与具有氧化还原活性的胱氨酸二硫键/半胱氨酸二硫醇的小蛋白质超家族中的硫氧还蛋白有远缘同源性。
Eur J Biochem. 1983 Oct 17;136(1):223-32. doi: 10.1111/j.1432-1033.1983.tb07730.x.
6
7
Evidence for use of rare codons in the dnaG gene and other regulatory genes of Escherichia coli.大肠杆菌dnaG基因及其他调控基因中稀有密码子使用的证据。
Proc Natl Acad Sci U S A. 1983 Feb;80(3):687-91. doi: 10.1073/pnas.80.3.687.
8
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Methods Enzymol. 1983;101:20-78. doi: 10.1016/0076-6879(83)01005-8.
9
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10
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