Swain M A, Galloway D A
J Virol. 1983 Jun;46(3):1045-50. doi: 10.1128/JVI.46.3.1045-1050.1983.
We have determined the complete nucleotide sequence of the thymidine kinase gene of herpes simplex virus (HSV) type 2 strain 333. The sequence of the thymidine kinase gene exhibits an open translational reading frame of 1,128 nucleotides encoding a protein of 376 amino acids. The DNA sequence was compared with that of the HSV type 1 thymidine kinase gene from strain MP (S. L. McKnight, Nucleic Acids Res. 8:5949-5964, 1980) and from strain CL 101 (M. J. Wagner, J. A. Sharp, and W. C. Summers, Proc. Natl. Acad. Sci. U.S.A. 78:1441-1445, 1981) to assess the extent of intra- and intertypic variation for one viral gene. The nucleotides encoding the structural gene varied 1.7% between the two HSV type 1 strains and 19% between HSV type 1 and HSV type 2, which translated to differences in the amino acid sequence of the two proteins of 1.9 and 27%, respectively. The DNA encoding the 5' regulatory sequences appeared to be more conserved than the DNA coding for the structural gene, and the DNA at the 3' end of the gene was the least homologous.
我们已经确定了单纯疱疹病毒2型（HSV-2）333株胸苷激酶基因的完整核苷酸序列。胸苷激酶基因的序列呈现出一个1128个核苷酸的开放翻译阅读框，编码一个由376个氨基酸组成的蛋白质。将该DNA序列与来自MP株（S.L.麦克奈特，《核酸研究》8:5949 - 5964，1980）和CL 101株（M.J.瓦格纳、J.A.夏普和W.C.萨默斯，《美国国家科学院院刊》78:1441 - 1445，1981）的HSV-1胸苷激酶基因的序列进行比较，以评估一个病毒基因的型内和型间变异程度。编码结构基因的核苷酸在两个HSV-1株之间的差异为1.7%，在HSV-1和HSV-2之间的差异为19%，这分别转化为两种蛋白质氨基酸序列1.9%和27%的差异。编码5'调控序列的DNA似乎比编码结构基因的DNA更保守，并且基因3'端的DNA同源性最低。
