Jariwalla R J, Aurelian L, Ts'o P O
Proc Natl Acad Sci U S A. 1980 Apr;77(4):2279-83. doi: 10.1073/pnas.77.4.2279.
Transfection of Syrian hamster embryo cells with limit digests of Bgl II-, Hpa I-, or Bgl II/Hpa I-cleaved DNA from herpes simplex virus type 2 (strain S-1) but not with salmon sperm DNA resulted in the appearance of refractile, morphologically altered cells at a frequency of 10(-5)/0.005 microgram of viral DNA within two to four passages. Transformed lines manifested reduced serum requirement and anchorage-independent growth and were tumorigenic in newborn hamsters. They expressed ICP10, a viral protein immunologically identical to the cervical-tumor-associated AG-4 antigen. Transforming activity was localized in the 16.5 x 10(6)-dalton Bgl II/Hpa I double-digest fragment CDs-1, which exhibited sequence homology to the Bgl II/Hpa I fragment CD of DNA from herpes simplex virus type 2 strain 333, mapping between coordinates 0.43 and 0.58 on the physical map of strain 333 DNA. This fragment, CD333, was also shown to induce neoplastic transformation.
用来自2型单纯疱疹病毒（S-1株）经Bgl II、Hpa I或Bgl II/Hpa I酶切的有限消化产物转染叙利亚仓鼠胚胎细胞，但用鲑鱼精DNA转染则未出现这种情况，在两到四代内，每0.005微克病毒DNA会以10(-5)的频率出现折光性、形态改变的细胞。转化细胞系表现出对血清需求降低和不依赖贴壁生长，并且在新生仓鼠中具有致瘤性。它们表达ICP10，一种与宫颈肿瘤相关的AG-4抗原在免疫学上相同的病毒蛋白。转化活性定位于16.5×10(6)道尔顿的Bgl II/Hpa I双酶切片段CDs-1，该片段与2型单纯疱疹病毒333株DNA的Bgl II/Hpa I片段CD具有序列同源性，位于333株DNA物理图谱坐标0.43至0.58之间。该片段CD333也被证明可诱导肿瘤转化。
