Rubin D B, Drab E A, Ward W F, Smith L J, Fowell S M
Radiat Res. 1984 Aug;99(2):420-32.
Confluent monolayers of bovine aortic endothelial and smooth muscle cells were exposed to 0-5.0 Gy of 60Co gamma rays. From 0 to 72 hr after irradiation, the monolayer and culture medium were analyzed for cell (nuclei) number, DNA and protein content, the activities of angiotensin converting enzyme (ACE), lactate dehydrogenase (LDH), and superoxide dismutase (SOD), and LDH isoenzyme profile. Irradiated endothelial cells exhibited a time- and dose-dependent increase in cell detachment, decreased DNA and protein content and reduced ACE active per attached cell, increased LDH and SOD activities per microgram of DNA, and increased LDH activity in the culture medium. The latter was accompanied by a shift from LDH 1 to LDH 4 and 5. The release of LDH activity, observed after 0.5 Gy, was the most sensitive endothelial response, and occurred independent of or preceding cell detachment. Vascular smooth muscle cells contained two to three times more SOD activity than did endothelial cells and exhibited no significant responses to 5.0 Gy.
将牛主动脉内皮细胞和平滑肌细胞的融合单层暴露于0 - 5.0 Gy的60Coγ射线下。在照射后0至72小时内，对单层细胞和培养基进行分析，检测细胞（细胞核）数量、DNA和蛋白质含量、血管紧张素转换酶（ACE）、乳酸脱氢酶（LDH）和超氧化物歧化酶（SOD）的活性以及LDH同工酶谱。照射后的内皮细胞表现出细胞脱离的时间和剂量依赖性增加、DNA和蛋白质含量降低、每个贴壁细胞的ACE活性降低、每微克DNA的LDH和SOD活性增加以及培养基中LDH活性增加。后者伴随着从LDH 1向LDH 4和5的转变。在0.5 Gy照射后观察到的LDH活性释放是内皮细胞最敏感的反应，且发生在细胞脱离之前或与细胞脱离无关。血管平滑肌细胞的SOD活性比内皮细胞高两到三倍，并且对5.0 Gy没有明显反应。
