用锂、利血平、丙咪嗪以及锂与利血平或丙咪嗪联合长期治疗后，大鼠大脑皮层钙调蛋白含量无变化。
No change in rat cerebral cortex calmodulin content following chronic treatment with lithium, reserpine, imipramine, and lithium combined with reserpine or imipramine.
作者信息
Andersen P H, Geisler A, Klysner R
出版信息
Acta Pharmacol Toxicol (Copenh). 1984 May;54(5):394-9. doi: 10.1111/j.1600-0773.1984.tb01948.x.
Rats were treated 2-3 weeks with lithium, reserpine, imipramine, and combinations of lithium with reserpine or imipramine. Lithium was given in the diet, while the other drugs were dissolved in 0.9% saline and given intraperitoneally twice daily. The control and lithium groups received only vehicle injections. Twenty-four hours after the last injection the rats were decapitated and the cerebral cortex dissected. The tissue was sliced and the noradrenaline-stimulated cyclic AMP accumulation determined or the tissue was homogenized and centrifuged at 10,000 X g for 30 min. and the calmodulin content determined in the pellet and the supernatant. Reserpine treatment was found to cause an 50% increase in the noradrenaline-stimulated cyclic AMP accumulation, while treatment with imipramine and the combination lithium-imipramine decreased the noradrenaline-stimulated cyclic AMP accumulation by 40%. The tissue content of calmodulin was, however, found unaltered by all treatments.
用锂盐、利血平、丙咪嗪以及锂盐与利血平或丙咪嗪的组合对大鼠进行2 - 3周的治疗。锂盐通过饮食给予，而其他药物溶解在0.9%的盐水中，每天腹腔注射两次。对照组和锂盐组仅接受赋形剂注射。最后一次注射24小时后，将大鼠断头并解剖大脑皮层。将组织切片，测定去甲肾上腺素刺激的环磷酸腺苷（cAMP）积累，或者将组织匀浆并在10,000×g下离心30分钟，然后测定沉淀物和上清液中的钙调蛋白含量。发现利血平治疗导致去甲肾上腺素刺激的cAMP积累增加50%，而丙咪嗪治疗以及锂盐 - 丙咪嗪联合治疗使去甲肾上腺素刺激的cAMP积累减少40%。然而，发现所有治疗均未改变钙调蛋白的组织含量。
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