Aabo S, Olsen J E, Threlfall E J, Brown D J
Department of Veterinary Microbiology, Royal Veterinary and Agricultural University, Frederiksberg C., Denmark.
Res Microbiol. 1995 Nov-Dec;146(9):751-9. doi: 10.1016/0923-2508(96)81071-0.
Six wild-type (wt) strains of Salmonella typhimurium, one wt strain of S. heidelberg and 12 wt strains of Escherichia coli were isolated based on both hybridization to a 6-kb HindIII fragment of the non-virulence coding part of the S. dublin serovar-specific virulence plasmid and the absence of hybridization to the virulence genes (spv genes) of the same plasmid. Such hybridization was shown to be caused by resident plasmids in all strains and to involve the same region of 30 to 37 kb of consecutive HindIII fragments on the S. dublin virulence plasmid, suggesting a common origin of this plasmid DNA. Nine of the plasmids were selected for detailed characterization and were shown not to be of the same plasmid species. They varied in size between 44 and 88 kb, they showed incompatibility with the plasmid K-MP10, or belonged to incompatibility group X, and with the exception of five plasmids from E. coli, they showed different HindIII restriction profile patterns.
基于与都柏林沙门氏菌血清型特异性毒力质粒非毒力编码部分的6kb HindIII片段杂交，且与同一质粒的毒力基因（spv基因）无杂交，分离出6株鼠伤寒沙门氏菌野生型（wt）菌株、1株海德堡沙门氏菌wt菌株和12株大肠杆菌wt菌株。结果表明，所有菌株中的这种杂交均由常驻质粒引起，且涉及都柏林沙门氏菌毒力质粒上30至37kb连续HindIII片段的同一区域，提示该质粒DNA有共同起源。选择其中9个质粒进行详细表征，结果显示它们并非同一质粒种类。它们的大小在44至88kb之间，与质粒K-MP10不兼容，或属于不兼容群X，除了来自大肠杆菌的5个质粒外，它们显示出不同的HindIII限制性酶切图谱模式。
