Max Planck Institute for Molecular Cell Biology and Genetics, Dresden, Germany.
Excellence Cluster, Physics of Life, Technische Universität Dresden, Dresden, Germany.
EMBO Rep. 2022 Nov 7;23(11):e54025. doi: 10.15252/embr.202154025. Epub 2022 Sep 22.
Adenosine triphosphate (ATP) production and utilization is critically important for animal development. How these processes are regulated in space and time during tissue growth remains largely unclear. We used a FRET-based sensor to dynamically monitor ATP levels across a growing tissue, using the Drosophila larval wing disc. Although steady-state levels of ATP are spatially uniform across the wing pouch, inhibiting oxidative phosphorylation reveals spatial differences in metabolic behavior, whereby signaling centers at compartment boundaries produce more ATP from glycolysis than the rest of the tissue. Genetic perturbations indicate that the conserved Hedgehog signaling pathway can enhance ATP production by glycolysis. Collectively, our work suggests the existence of a homeostatic feedback loop between Hh signaling and glycolysis, advancing our understanding of the connection between conserved developmental patterning genes and ATP production during animal tissue development.
三磷酸腺苷（ATP）的产生和利用对动物的发育至关重要。在组织生长过程中，这些过程如何在空间和时间上被调控在很大程度上还不清楚。我们使用一种基于荧光共振能量转移（FRET）的传感器，通过监测果蝇幼虫翅膀盘的 ATP 水平，来研究这个问题。尽管在翅膀囊中，ATP 的稳态水平在空间上是均匀的，但抑制氧化磷酸化会揭示出代谢行为的空间差异，即信号中心在隔室边界处通过糖酵解产生的 ATP 比组织的其他部分多。遗传干扰表明，保守的 Hedgehog 信号通路可以通过糖酵解来增强 ATP 的产生。总的来说，我们的工作表明了 Hh 信号和糖酵解之间存在一个动态平衡的反馈回路，这为我们理解在动物组织发育过程中，保守的发育模式基因与 ATP 产生之间的联系提供了新的认识。
