Trowsdale J, Kelly A, Lee J, Carson S, Austin P, Travers P
Cell. 1984 Aug;38(1):241-9. doi: 10.1016/0092-8674(84)90546-4.
Three overlapping cosmid clones contain coding sequences for four HLA Class II genes, provisionally identified as two HLA-SB alpha and two HLA-SB beta genes. The genes are in the order beta, alpha, beta, alpha, inverted with respect to each other. One of the SB beta genes contains a 513 bp sequence that appears to be a processed pseudogene, flanked by direct 17 bp repeat sequences, in the intron upstream of the beta 1 exon. The pseudogene is homologous to a family of sequences of approximately 25-40 members, most of which are not on chromosome 6. A cDNA clone, highly homologous to the pseudogene, except for its 5' end, contains a normal poly(A) addition site and a poly(A) tail. The cDNA clone is homologous to a single-copy gene in both man and mouse, encoded on human chromosome 15. A search of published DNA sequences identified a mouse sequence, with about 77% similarity to the pseudogene sequence, in the negative strand of an intron in a mouse dihydrofolate reductase gene. The second SB beta gene does not contain the pseudogene sequence.
三个重叠的黏粒克隆包含四个HLA II类基因的编码序列，暂定为两个HLA - SBα基因和两个HLA - SBβ基因。这些基因的排列顺序为β、α、β、α，彼此呈反向排列。其中一个SBβ基因在β1外显子上游的内含子中含有一段513 bp的序列，该序列似乎是一个加工过的假基因，两侧有17 bp的直接重复序列。该假基因与一个约有25 - 40个成员的序列家族同源，其中大多数不在6号染色体上。一个cDNA克隆，除了其5'端外，与该假基因高度同源，含有一个正常的多聚腺苷酸添加位点和一个多聚腺苷酸尾巴。该cDNA克隆在人和小鼠中均与一个单拷贝基因同源，该单拷贝基因位于人类15号染色体上。对已发表的DNA序列进行搜索，在小鼠二氢叶酸还原酶基因内含子的负链中发现了一个与假基因序列相似度约为77%的小鼠序列。第二个SBβ基因不包含假基因序列。
