[由质粒Rms163诱导的噬菌体转座子B39对铜绿假单胞菌细胞溶原化的刺激作用]

[Stimulation of the lysogenization of Pseudomonas aeruginosa cells by phage transposon B39 induced by plasmid Rms163].

作者信息

Gerasimov V A, Eremenko E N, Khrenova E A, Gorbunova S A, Krylov V N

出版信息

Genetika. 1984 Jul;20(7):1080-7.

Abstract

The influence of Rms163 plasmid on lysogenization of Pseudomonas aeruginosa cells by B39 phage was studied. Plasmid Rms163 was shown to increase the frequency of lysogenization of PAO1 cells 7-8 times. C-mutants of B39 phage were isolated. According to complementation test, c-mutants were distributed into two groups--cI and cII/III. The product of cI is essential for establishment and maintenance of lysogenic state, cII/cIII product being only necessary for establishment of lysogenization. The mutants with special characteristics were isolated: B39cx1 phage carries a mutation which seems to be located on a regulatory site essential for establishment of lysogenic state. The region of the B39 genome responsible for interaction with Rms163 plasmid was mapped. Possible mechanisms of Rms163 plasmid interference with transposable B39 phage are discussed.

摘要

研究了Rms163质粒对B39噬菌体使铜绿假单胞菌细胞溶源化的影响。结果表明，质粒Rms163可使PAO1细胞的溶源化频率提高7 - 8倍。分离出了B39噬菌体的C突变体。根据互补试验，C突变体分为两组——cI和cII/III。cI产物对于溶源状态的建立和维持至关重要，而cII/cIII产物仅对溶源化的建立是必需的。分离出了具有特殊特性的突变体：B39cx1噬菌体携带一个似乎位于溶源状态建立所必需的调控位点上的突变。绘制了B39基因组中负责与Rms163质粒相互作用的区域。讨论了Rms163质粒干扰转座B39噬菌体的可能机制。