Gorelyshev A S, Kul'ba A M, Tsygankov Iu D, Fomichev Iu K
Genetika. 1985 Nov;21(11):1782-6.
76 mutants with impaired ability to lysogenize host cells were isolated in SM phage after mutagenesis using several chemical mutagens. By means of complementation test, these mutants were distributed into two groups, cI and cII. The mutants of the cI group were similar phenotypically to the cI mutants of phage lambda defective in synthesis of repressor. The mutants of the cII group establish and support the lysogenic state in infected cells with very low frequency. Temperature-sensitive mutants belonging to 13 complementation groups and nonlysogenizing mutants of the cI and cII groups were used in genetic mapping of SM phage. Mutual positions of markers and relative distances between them were determined by the method of two-factorial crosses. The greatest distance equal to 20 units of recombination was determined between ts 88 marker and one of early genes marked with ts 105 mutation. The genes cI and cII are closely linked to each other and also to ts 105 marker and are situated at one end of the genetic map.
使用几种化学诱变剂诱变后，在SM噬菌体中分离出76个溶源化宿主细胞能力受损的突变体。通过互补试验，这些突变体被分为两组，即cI和cII。cI组的突变体在表型上与阻遏物合成缺陷的λ噬菌体的cI突变体相似。cII组的突变体在感染细胞中以极低的频率建立并维持溶源状态。属于13个互补群的温度敏感突变体以及cI和cII组的非溶源化突变体被用于SM噬菌体的基因定位。标记的相互位置及其之间的相对距离通过双因子杂交法确定。在ts 88标记与标记为ts 105突变的一个早期基因之间确定了最大距离，等于20个重组单位。基因cI和cII彼此紧密连锁，也与ts 105标记紧密连锁，位于遗传图谱的一端。
