Zaccone G, Fasulo S, Lo Cascio P, Licata A, Ainis L, Affronte R
Department of Animal Biology and Marine Ecology, Messina University, Italy.
Histochemistry. 1987;87(5):431-8. doi: 10.1007/BF00496814.
The surface epidermis of Ambystoma tigrinum larvae was examined at the light- and electron-microscope levels using five different lectin conjugates as probes for the detection of sugar residues on the cell membranes. Concanavalin A (Con-A), wheat-germ agglutinin (WGA), Ricinus communis agglutinin I (RCA-I), Dolichos biflorus agglutinin and soybean agglutinin (SBA) conjugates clearly labelled the surface cells, especially their apical surfaces. At electron microscopy, the labelling on plasma membranes was found to exhibit regional differences. Among the lectins tested WGA displayed a particularly characteristic binding pattern. WGA also bound to basolateral cell surfaces, including the tight-junction zone which was also stained by the RCA-I conjugate. The different labelling intensity and staining patterns obtained with the conjugates indicated the polarity of the cell surfaces. It is also assumed that the WGA staining of the basolateral membranes and intercellular spaces reflected transcellular transport, which is facilitated by acidic glycoconjugates. Other functional aspects of the polarized distribution of the lectin conjugates were also correlated with the receptor sites of certain sugar residues.
使用五种不同的凝集素缀合物作为检测细胞膜上糖残基的探针，在光学显微镜和电子显微镜水平上对虎纹钝口螈幼虫的表面表皮进行了检查。伴刀豆球蛋白A（Con-A）、麦胚凝集素（WGA）、蓖麻凝集素I（RCA-I）、双花扁豆凝集素和大豆凝集素（SBA）缀合物清晰地标记了表面细胞，尤其是它们的顶端表面。在电子显微镜下，发现质膜上的标记呈现出区域差异。在所测试的凝集素中，WGA显示出特别典型的结合模式。WGA还与基底外侧细胞表面结合，包括也被RCA-I缀合物染色的紧密连接区。用缀合物获得的不同标记强度和染色模式表明了细胞表面的极性。还推测基底外侧膜和细胞间空间的WGA染色反映了跨细胞转运，这由酸性糖缀合物促进。凝集素缀合物极化分布的其他功能方面也与某些糖残基的受体位点相关。
