枯草芽孢杆菌spoIIA和spoVA基因座在噬菌体phi 105DI:1t中的克隆。
Cloning of the Bacillus subtilis spoIIA and spoV A loci in phage phi 105DI:1t.
作者信息
Savva D, Mandelstam J
出版信息
J Gen Microbiol. 1984 Aug;130(8):2137-45. doi: 10.1099/00221287-130-8-2137.
PMID:6088673
Abstract
A 6.95 kb HindIII-generated DNA fragment from Bacillus subtilis 168 was inserted into the DNA of phage phi 105DI:1t. The recombinant phage (phi 105DS1) contained DNA of 33.8 kb as compared with 35.2 kb for phi 105DI:1t and 39.2 kb for the wild-type phage. In the presence of helper phage, phi 105DS1 complemented both spoIIA and spoV A mutations in B. subtilis.
摘要
将来自枯草芽孢杆菌168的一段经HindIII酶切产生的6.95 kb DNA片段插入噬菌体phi 105DI:1t的DNA中。重组噬菌体（phi 105DS1）含有33.8 kb的DNA，相比之下，phi 105DI:1t为35.2 kb，野生型噬菌体为39.2 kb。在辅助噬菌体存在的情况下，phi 105DS1可互补枯草芽孢杆菌中的spoIIA和spoVA突变。
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