Jenkinson H F, Mandelstam J
J Gen Microbiol. 1983 Jul;129(7):2229-40. doi: 10.1099/00221287-129-7-2229.
The lys gene of Bacillus subtilis was inserted into prophage phi 105. The recombinant phage (phi 105dlys) contained DNA which was about 2 MDal smaller than the wild-type phage DNA, and the phage particles had no tails. The phage did not plaque but, when provided with tails in vitro, it transduced both lys-1 and lys-3 strains of B. subtilis to Lys$. The lys$ gene was located on a 2.5 MDal EcoRI restriction fragment. Subsequently this phage was phi 105 105dspoIIIB, was also defective, i.e. without tails. The DNA was 1.5 MDal smaller than the wild-type phage DNA and the spoIIIB2$ gene was located on a 3 MDal EcoRI fragment. When provided with tails in vitro, phage phi 105dspoIIIB transduced cells of a spoIIIB2 recipient to Spo$. In these transductants the spoIIIB2 mutation was complemented, and the cells sporulated normally.
将枯草芽孢杆菌的lys基因插入原噬菌体phi 105中。重组噬菌体（phi 105dlys）所含的DNA比野生型噬菌体DNA小约2 MDal，且噬菌体颗粒无尾部。该噬菌体不能形成噬菌斑，但在体外提供尾部时，它能将枯草芽孢杆菌的lys - 1和lys - 3菌株转导为Lys$。Lys$基因位于一个2.5 MDal的EcoRI限制性片段上。随后该噬菌体phi 105 105dspoIIIB也有缺陷，即无尾部。其DNA比野生型噬菌体DNA小1.5 MDal，spoIIIB2$基因位于一个3 MDal的EcoRI片段上。在体外提供尾部时，噬菌体phi 105dspoIIIB将spoIIIB2受体细胞转导为Spo$。在这些转导子中，spoIIIB2突变得到互补，细胞能正常形成芽孢。
