Okada Y, Richardson M A, Ikegami N, Nomoto A, Furuichi Y
J Virol. 1984 Sep;51(3):856-9. doi: 10.1128/JVI.51.3.856-859.1984.
The complete nucleotide sequence of human rotavirus (Wa strain) genome segment 10 was determined by using a cloned DNA copy. The sequence data indicated that segment 10 is A + T rich (65%) and consists of 750 base pairs. The positive strand of segment 10 contains a single open reading frame that extends 175 codons from the first AUG triplet (residues 42 through 44). The amino acid sequence of the segment 10 product was deduced from the nucleotide sequence. There are two distinct glycosylation sites at the N-terminal hydrophobic region, consistent with previous findings that this protein exists in a glycosylated form. The apparent molecular weight (20,000) of the unglycosylated, precursor polypeptide is in good agreement with the one calculated from the predicted amino acid sequence. Structural analysis of the positive strand (mRNA from segment 10) showed that it could form, like mRNA from segment 11, a stable panhandle structure involving the 5' and 3'-terminal regions. The nucleotide sequence of segment 10 from simian rotavirus, recently determined by Both et al. (J. Virol. 48:335-339, 1983) was found to be highly homologous to, and to share several important features with, segment 10 of human rotavirus.
利用克隆的DNA拷贝测定了人轮状病毒（Wa株）基因组第10节段的完整核苷酸序列。序列数据表明第10节段富含A+T（65%），由750个碱基对组成。第10节段的正链包含一个单一的开放阅读框，从第一个AUG三联体（第42至44位残基）延伸175个密码子。从核苷酸序列推导了第10节段产物的氨基酸序列。在N端疏水区域有两个不同的糖基化位点，这与该蛋白以糖基化形式存在的先前发现一致。未糖基化的前体多肽的表观分子量（20,000）与根据预测的氨基酸序列计算出的分子量非常吻合。对正链（第10节段的mRNA）的结构分析表明，它可以像第11节段的mRNA一样，形成一个涉及5'和3'末端区域的稳定的柄状结构。Both等人（《病毒学杂志》48:335 - 339, 1983）最近测定的猿猴轮状病毒第10节段的核苷酸序列与人轮状病毒第10节段高度同源，并具有几个重要特征。
