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富含生长因子的可注射纤维蛋白支架用于皮肤修复。

An Injectable Fibrin Scaffold Rich in Growth Factors for Skin Repair.

机构信息

Department of Stomatology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, China.

School of Chemistry and Chemical Engineering, Shanghai Key Laboratory of Electrical Insulation and Thermal Aging, Shanghai Jiao Tong University, Shanghai 200240, China.

出版信息

Biomed Res Int. 2021 Feb 8;2021:8094932. doi: 10.1155/2021/8094932. eCollection 2021.

DOI:10.1155/2021/8094932
PMID:33628814
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7884109/
Abstract

Platelet aggregates, such as PRP, PRF, and CGF, have been used alone or in combination with other grafting materials to enhance restoration outcomes. The process for preparing these autografting materials requires two-step centrifugation or specific centrifuges. In this study, we obtained an injectable fibrin scaffold (IFS) rich in growth factors by one-step centrifugation of whole blood from rabbits. The purpose of this study is to introduce some characteristics of IFS. This scaffold was characterized using various techniques, including Masson's trichrome staining, scanning electron microscopy, porosity measurements, and cell counting. The sustained release of growth factors, including PDGF, VEGF, TGF-1, IGF, FGF, and EGF, was quantified using ELISA assay. The obtained IFS was tested for its effects on cell proliferation, extracellular matrix deposition, and full-thickness skin defect repair. The prepared IFS is characterized by a loose fibrin network structure with white blood cells and platelets that slowly release growth factors and can promote the healing of skin defects via the promotion of cell proliferation, collagen deposition, and tissue revascularization. In addition, its liquid properties and porous structure are conducive to its application as a therapeutic component in tissue engineering.

摘要

血小板聚集物，如 PRP、PRF 和 CGF，已被单独或与其他移植物材料联合使用，以增强修复效果。制备这些自体移植物材料的过程需要两步离心或特定的离心机。在这项研究中，我们通过对兔全血进行一步离心获得了富含生长因子的可注射纤维蛋白支架（IFS）。本研究的目的是介绍 IFS 的一些特性。该支架通过各种技术进行了表征，包括 Masson 三色染色、扫描电子显微镜、孔隙率测量和细胞计数。使用 ELISA 测定法定量测定了包括 PDGF、VEGF、TGF-1、IGF、FGF 和 EGF 在内的生长因子的持续释放。通过细胞增殖、细胞外基质沉积和全厚皮肤缺损修复来测试所制备的 IFS 的效果。所制备的 IFS 的特点是具有白细胞和血小板的松散纤维蛋白网络结构，这些白细胞和血小板缓慢释放生长因子，并通过促进细胞增殖、胶原蛋白沉积和组织再血管化来促进皮肤缺损的愈合。此外，其液体特性和多孔结构有利于将其作为组织工程中的治疗成分应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d145/7884109/5461c4c9d9b9/BMRI2021-8094932.011.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d145/7884109/5461c4c9d9b9/BMRI2021-8094932.011.jpg

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