Luotola J
Med Biol. 1984;62(2):115-8.
Irradiation experiments were performed with Ehrlich ascites tumour cells grown in the peritoneal cavity of mice. A 20 MeV proton beam was used as the source of ionizing radiation. The generation of free radicals was investigated with EPR spectrometry. Lipid peroxidation products were determined by the thiobarbiturate reaction, UV-absorption, and fluorometric measurements. Membrane injuries were visualized by electron microscopy. Free radicals (like H and OH) were formed in the cells during the irradiation. Radical reactions were transferred to membrane lipids producing secondary radicals, diene conjugation, malondialdehyde formation and fluorescent end products. By using electron microscopy severe damage could be detected in cell membranes.
使用在小鼠腹腔中生长的艾氏腹水瘤细胞进行了辐照实验。使用20 MeV的质子束作为电离辐射源。通过电子顺磁共振光谱法研究自由基的产生。通过硫代巴比妥酸反应、紫外吸收和荧光测量来测定脂质过氧化产物。通过电子显微镜观察膜损伤情况。辐照过程中细胞内形成了自由基（如H和OH）。自由基反应转移至膜脂，产生次级自由基、二烯共轭、丙二醛形成和荧光终产物。通过电子显微镜可检测到细胞膜受到严重损伤。
