Balk S D, Gunther H S, Morisi A
Life Sci. 1984 Sep 10;35(11):1157-71. doi: 10.1016/0024-3205(84)90186-3.
Normal chicken heart mesenchymal cells at low density in monolayer culture in plasma-containing medium have a polygonal shape and are proliferatively quiescent. The combination of epidermal growth factor and insulin at hyperphysiological concentration, an insulin-like growth factor surrogate, causes these cells to assume a fusiform shape and to increase 40-fold in number during four days of incubation. These mitogenic hormones do not, however, induce normal chicken heart mesenchymal cells to form colonies in agarose suspension culture. Chicken heart mesenchymal cells infected with the Schmidt-Ruppin or Prague-A strains of Rous sarcoma virus or with the Fujinami or Y73 avian sarcoma viruses assume spindle and round shapes, increase 50-100 fold in number during four days of monolayer culture in the absence of mitogenic hormones and form macroscopic colonies during 3-4 days of agarose suspension culture. The autonomous (mitogenic hormone-independent) proliferation, in monolayer culture, of cells infected with temperature-sensitive transformation mutants of Rous sarcoma virus (tsNY68, tsNY72, tsLA24, tsLA29) is temperature-sensitive. Chicken heart mesenchymal cells infected with avian erythroblastosis virus assume spindle shapes and proliferate in monolayer culture at a rate comparable to that of sarcoma virus-infected cells but do not, however, form colonies in agarose suspension culture. Cells infected with the myelocytomatosis virus MC29 assume stellate shapes and increase 18-fold in number during four days of monolayer culture. Cells infected with the myelocytomatosis virus MH2 assume fusiform shapes and increase fourfold in number during four days of monolayer culture. Neither MC29 nor MH2 renders chicken heart mesenchymal cells capable of colony formation in agarose suspension culture. Infection with avian leukosis viruses (RAV-1, RAV-2, RPL-42) or with transformation-defective mutants of Rous sarcoma virus (tdNY105, 107, 109) does not affect the morphology or proliferative behavior of chicken heart mesenchymal cells. Monolayer culture of chicken heart mesenchymal cells in plasma-containing medium appears, therefore, to define the ability of onc genes of acute transforming avian retroviruses to induce autonomous (mitogenic hormone-independent) cell proliferation, the essential characteristic of neoplasia. The differences in transformed morphology and rates of autonomous proliferation between cells infected with different acute transforming retroviruses probably reflects differences in the modes of action of the transforming proteins encoded by the onc genes of the respective viruses.(ABSTRACT TRUNCATED AT 400 WORDS)
在含血浆培养基中进行单层培养的低密度正常鸡心脏间充质细胞呈多边形，增殖静止。超生理浓度的表皮生长因子和胰岛素（一种胰岛素样生长因子替代物）组合，可使这些细胞呈梭形，并在培养四天后数量增加40倍。然而，这些促有丝分裂激素不会诱导正常鸡心脏间充质细胞在琼脂糖悬浮培养中形成集落。感染施密特-鲁平或布拉格-A株劳斯肉瘤病毒、藤浪或Y73禽肉瘤病毒的鸡心脏间充质细胞呈纺锤形和圆形，在无促有丝分裂激素的情况下进行单层培养四天，数量增加50 - 100倍，并在琼脂糖悬浮培养3 - 4天形成肉眼可见的集落。感染劳斯肉瘤病毒温度敏感转化突变体（tsNY68、tsNY72、tsLA24、tsLA29）的细胞在单层培养中的自主（不依赖促有丝分裂激素）增殖是温度敏感的。感染禽成红细胞增多症病毒的鸡心脏间充质细胞呈纺锤形，在单层培养中的增殖速率与感染肉瘤病毒的细胞相当，但在琼脂糖悬浮培养中不形成集落。感染髓细胞瘤病毒MC29的细胞呈星状，在单层培养四天后数量增加18倍。感染髓细胞瘤病毒MH2的细胞呈梭形，在单层培养四天后数量增加四倍。MC29和MH2均未使鸡心脏间充质细胞能够在琼脂糖悬浮培养中形成集落。感染禽白血病病毒（RAV - 1、RAV - 2、RPL - 42）或劳斯肉瘤病毒转化缺陷突变体（tdNY105、107、109）不影响鸡心脏间充质细胞的形态或增殖行为。因此，在含血浆培养基中对鸡心脏间充质细胞进行单层培养似乎可以确定急性转化禽逆转录病毒的癌基因诱导自主（不依赖促有丝分裂激素）细胞增殖的能力，这是肿瘤形成的基本特征。感染不同急性转化逆转录病毒的细胞在转化形态和自主增殖速率上的差异可能反映了各自病毒癌基因编码的转化蛋白作用方式的差异。（摘要截于400字）
